Transmission FT-IR Chemical Imaging on Glass Substrates: Applications in Infrared Spectral Histopathology
Transmission FT-IR Chemical Imaging on Glass Substrates: Applications in Infrared Spectral Histopathology
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DOI：
10.1021/ac403412n
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发表时间：
2014-02-04
影响因子：
7.4
通讯作者：
Gardner, Peter
中科院分区：
文献类型：
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作者：
Bassan, Paul;Mellor, Joe;Gardner, Peter
传统上，透射模式下的傅里叶变换红外 (FT-IR) 化学成像是在昂贵的中红外透明窗口（例如氟化钡/氟化钙）上进行的，这些窗口比玻璃更脆弱，使得组织病理学实验室的准备工作更加繁琐。这里提出了一种解决方案，使用廉价的玻璃基板进行 FT-IR 化学成像，该基板具有高波数传输窗口，可以测量大约 100°C 处发生的 C-H、N-H 和 O-H 拉伸。 2500-3800 厘米(-1)。无法获得低于 1800 cm(-1) 的红外光谱“指纹”区域；然而，我们使用乳腺组织微阵列 (TMA) 上的 71 名患者作为研究模型，证明高波数范围中包含大量信息。重要的是，我们证明组织可以分为四种基本组织细胞类型，并且仅使用上皮细胞，可以合理区分正常和正常细胞。 可以发现恶性组织。
Fourier transform-infrared (FT-IR) chemical imaging in transmission mode has traditionally been performed on expensive mid-IR transparent windows such as barium/calcium fluoride, which are more fragile than glass, making preparation in the histopathology laboratories more cumbersome. A solution is presented here by using cheap glass substrates for the FT-IR chemical imaging, which has a high-wavenumber transmission window allowing measurement of the C-H, N-H, and O-H stretches occurring at ca. 2500-3800 cm(-1). The ''fingerprint" region of the IR spectrum occurring below 1800 cm(-1) is not obtainable; however, we demonstrate that a wealth of information is contained in the high wavenumber range using 71 patients on a breast tissue microarray (TMA) as a model for investigation. Importantly, we demonstrate that the tissue can be classified into four basic tissue cell types and that using just the epithelial cells, reasonable discrimination of normal and malignant tissue can be found.