Comparison of the surface coat proteins of the pine wood nematode appeared during host pine infection and in vitro culture by a proteomic approach.
Comparison of the surface coat proteins of the pine wood nematode appeared during host pine infection and in vitro culture by a proteomic approach.
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DOI：
10.1094/phyto-04-10-0109
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发表时间：
2010-11
期刊：
影响因子：
3.2
通讯作者：
R. Shinya;Hironobu Morisaka;Y. Takeuchi;M. Ueda;K. Futai
中科院分区：
文献类型：
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作者：
R. Shinya;Hironobu Morisaka;Y. Takeuchi;M. Ueda;K. Futai
由松材线虫引起的松材线虫病是近年来世界范围内的检疫性病害。在这里，我们公开了被认为是在松树枯萎病发展的关键组成部分之一的PWN的表面涂层（SC）蛋白。这是第一个报告，专注于SC蛋白，并彻底确定了这些蛋白质的植物寄生线虫使用蛋白质组学方法。在这项研究中，SC蛋白质谱进行了比较PWNs上生长的真菌灰葡萄孢和宿主松树幼苗。结果表明，在感染过程中，松材线虫SC蛋白的总量急剧增加。37条蛋白质条带显示真菌生长和宿主来源的PWN之间的显著数量差异，并用于通过基质辅助激光解吸电离飞行时间质谱分析进行鉴定。这些包括几种被认为参与宿主免疫反应的蛋白质;例如，活性氧（ROS）的调节剂和ROS清除剂。这些结果可能表明PWN SC蛋白在调节或逃避宿主免疫应答中是至关重要的。我们的数据提供了一个新的见解松树枯萎病的机制和SC蛋白的生物学作用的植物寄生线虫。
Pine wilt disease, caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus, has become of worldwide quarantine concern in recent years. Here, we disclosed the surface coat (SC) proteins of the PWN which are thought to be one of the key components in pine wilt development. This is the first report that focused on the SC proteins and thoroughly identified those proteins of a plant-parasitic nematode using the proteomic approach. In this study, SC protein profiles were compared for PWNs grown on the fungus Botrytis cinerea and in host pine seedlings. The results demonstrated that the gross amount of PWN SC proteins drastically increased during infection of the host pine. Thirty-seven protein bands showed significant quantity differences between fungus-grown and host-origin PWNs, and were used for identification by matrix-assisted laser desorption ionization time of flight mass spectrometry analysis. These included several proteins that are presumed to be involved in the host immune response; for example, regulators of reactive oxygen species (ROS) and a ROS scavenger. These results might suggest that the PWN SC proteins are crucial in modulating or evading host immune response. Our data provide a new insight into the mechanism of pine wilt disease and the biological role of the SC proteins of plant-parasitic nematodes.