Optimizing culture conditions for free-living stages of the nematode parasite Strongyloides ratti
Optimizing culture conditions for free-living stages of the nematode parasite Strongyloides ratti
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DOI：
10.1016/j.exppara.2016.06.005
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发表时间：
2016-09-01
影响因子：
2.1
通讯作者：
Streit, Adrian
中科院分区：
文献类型：
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作者：
Dulovic, Alex;Puller, Vadim;Streit, Adrian
鼠寄生圆线虫Strongyloides ratti（S. Ratti）最近作为寄生虫生物学和进化的各个方面的模型系统出现。除了寄生孤雌生殖的雌性外，该物种还可以形成有性生殖的成年人的兼性自由生活世代。这些自由生活的蠕虫是食菌性的，在宿主的粪便中培养时生长良好。然而，在粪便培养中，很难找到蠕虫进行观察和实验操作。因此，也尝试提高S。在线虫生长培养基（NGM）平板上用大肠杆菌OP 50作为食物培养ratti，完全如对模式线虫秀丽隐杆线虫（Caelophabditis elegans）所述。为了提高培养成功率，我们测试了偶尔用于C. elegans，从表现最好的一个开始，系统地改变板组成、温度和食物，以进一步优化条件。在这里，我们提出了一个平板培养方案的自由生活阶段的S。ratti具有显著改善的繁殖成功率和寿命。(C)2016 Elsevier Inc. All rights reserved.
The rat parasitic nematode Strongyloides ratti (S. ratti) has recently emerged as a model system for various aspects of parasite biology and evolution. In addition to parasitic parthenogenetic females, this species can also form facultative free-living generations of sexually reproducing adults. These free-living worms are bacteriovorous and grow very well when cultured in the feces of their host. However, in fecal cultures the worms are rather difficult to find for observation and experimental manipulation. Therefore, it has also been attempted to raise S. ratti on Nematode Growth Media (NGM) plates with Escherichia coli OP50 as food, exactly as described for the model nematode Caenorhabditis elegans.Whilst worms did grow on these plates, their longevity and reproductive output compared to fecal cultures were dramatically reduced. In order to improve the culture success we tested other plates occasionally used for C. elegans and, starting from the best performing one, systematically varied the plate composition, the temperature and the food in order to further optimize the conditions. Here we present a plate culturing protocol for free-living stages of S. ratti with strongly improved reproductive success and longevity. (C) 2016 Elsevier Inc. All rights reserved.