Synthesis and characterization of tricalcium phosphate with Zn and Mg based dopants
Synthesis and characterization of tricalcium phosphate with Zn and Mg based dopants
复制标题
DOI：
10.1007/s10856-008-3395-4
复制
发表时间：
2008-07-01
影响因子：
3.7
通讯作者：
Bose, Susmita
中科院分区：
文献类型：
--
作者：
Xue, Weichang;Dahlquist, Kelli;Bose, Susmita
采用镁（Mg）和锌（Zn）双掺杂的方法制备磷酸三钙（TCP）陶瓷，并研究掺杂对TCP陶瓷物理、力学和生物学性能的影响。使用沉淀法合成具有1重量% Mg、0.3重量% Zn和双掺杂剂的TCP。表征了相组成和显微结构。考察了其力学性能和体外溶出行为。人成骨细胞培养用于确定掺杂剂对细胞-材料相互作用的影响。XRD分析表明，Mg掺杂的TCP在1250 ℃烧结后，出现了由β-TCP向α-TCP的延迟相变，得到了纯β-TCP相。Mg的加入改善了TCP的致密化行为。压缩强度也从24.0 MPa提高到77.2 MPa。此外，镁添加剂降低TCP在体外的溶解度。成骨细胞培养研究表明，镁的存在下，稳定的细胞材料界面，从而提高细胞的附着和生长。Zn掺杂的TCP具有良好的生物活性，可促进细胞分化和碱性磷酸酶（ALP）的表达。最高的细胞增殖和ALP表达被发现在双重Mg和Zn掺杂的TCP上。结果表明，Mg和Zn掺杂剂对改善TCP的力学性能和细胞-材料相互作用起着重要作用。这项工作还证明了双重Mg和Zn掺杂的TCP在骨科和牙科中使用的潜力，其显示出高机械强度，低吸收和改善的细胞-材料相互作用。
The purpose of this study is to prepare tricalcium phosphate (TCP) ceramic by dual dopants of magnesium (Mg) and zinc (Zn), and investigate the influence of dopants on the physical, mechanical and biological properties of TCP. TCP were synthesized with 1 wt% Mg, 0.3 wt% Zn and dual dopants using the precipitation process. Phase composition and microstructures were characterized. Mechanical properties and dissolution behavior in vitro were investigated. Human osteoblast cell culture was used to determine the influence of dopants on cell-materials interactions. XRD analysis indicated that Mg delayed phase transformation from beta to alpha-TCP and pure beta-TCP phase was obtained for Mg-doped TCP after sintered at 1250 degrees C. Addition of Mg improved densification behavior of TCP. Compression strength also increased from 24.0 MPa to 77.2 MPa after doping with Mg and Zn. Furthermore, Mg additive reduced the solubility of TCP in vitro. Osteoblast culture studies indicated that the presence of Mg stabilized the cell-material interface and thus improved cell attachment and growth. Zn-doped TCP exhibited good bioactivity, which enhanced cell differentiation and alkaline phosphatase (ALP) expression. The highest cell proliferation and ALP expression were found on dual Mg and Zn doped TCP. The results indicate that Mg and Zn dopants play a significant role towards improving mechanical properties and cell-materials interactions of TCP. This work also demonstrates the potential for dual Mg and Zn doped TCP to be used in orthopedics and dentistry, which displays high mechanical strength, low resorption and improved cell-material interaction.