3D Printing of Regenerated Silk Fibroin and Antibody-Containing Microstructures via Multiphoton Lithography
3D Printing of Regenerated Silk Fibroin and Antibody-Containing Microstructures via Multiphoton Lithography
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DOI：
10.1021/acsbiomaterials.7b00338
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发表时间：
2017-09-01
影响因子：
5.8
通讯作者：
Naik, Rajesh R.
中科院分区：
文献类型：
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作者：
Dickerson, Matthew B.;Dennis, Patrick B.;Naik, Rajesh R.
再生丝素是一种从蚕茧中提取的生物聚合物，由于其具有诱人的生化特性和可加工性，已被广泛应用于许多领域(如药物输送、组织修复、生物兼容电子衬底和光学)。在这里，我们报告了通过多光子光刻技术自由打印基于丝绸的3D微结构。利用多光子光刻，结合特定的光引发剂化学和印后交联，实现了包括自支撑丝素拱门在内的许多微结构。此外，通过打印丝素/免疫球蛋白水溶液，可以直接生产高保真和生物功能蛋白质结构。
Regenerated silk fibroin, a biopolymer derived from silkworm cocoons, is a versatile material that has been widely explored for a number of applications (e.g., drug delivery, tissue repair, biocompatible electronics substrates, and optics) due to its attractive biochemical properties and processability. Here, we report on the free-form printing of silk-based, 3D microstructures through multiphoton lithography. Utilizing multiphoton lithography in conjunction with specific photoinitiator chemistry and postprint cross-linking, a number of microarchitectures were achieved including self-supporting fibroin arches. Further, the straightforward production of high fidelity and biofunctional protein architectures was enabled through the printing of aqueous fibroin/immunoglobulin solutions.