Identification of the bacteriocin produced by cheese isolate Lactobacillus paraplantarum FT259 and its potential influence on Listeria monocytogenes biofilm formation
Identification of the bacteriocin produced by cheese isolate Lactobacillus paraplantarum FT259 and its potential influence on Listeria monocytogenes biofilm formation
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DOI：
10.1016/j.lwt.2015.06.014
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发表时间：
2015-12-01
影响因子：
6
通讯作者：
De Martinis, Elaine C. P.
中科院分区：
文献类型：
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作者：
Winkelstroeter, Lizziane Kretli;Tulini, Fabricio Luiz;De Martinis, Elaine C. P.
乳酸菌（LAB）以其技术特性而闻名，如抑制腐败和致病菌。本研究的目的是：(1)利用培养法和荧光原位杂交法（FISH）评价植物旁乳杆菌FT259对单核增生李斯特菌生物膜形成的影响；(2)利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）对该菌株从巴西半硬奶酪中分离得到的细菌素进行部分表征。我们还利用引物对plantaricin NC8、plantaricin S和plantaricin W结构基因进行聚合酶链反应，然后进行DNA测序，以确定产生的细菌素。显微镜观察结果表明，两种菌株共同培养形成的生物膜结构复杂且异质性。SDS-PAGE分析表明，部分纯化的细菌素分子量约为3900 Da， PCR-DNA测序检测到植物素NC8基因。据我们所知，这是第一次报道一种拟植物乳杆菌菌株对单核增生L的生物膜的抑制作用，以及该LAB物种对生物膜的形成。此外，我们已经证实L paraplantarum FT259含有产生植物素NC8的基因，这可能与观察到的抗菌活性有关。(C) 2015 Elsevier Ltd.版权所有。
Lactic acid bacteria (LAB) are known by their technological properties, such as the inhibition of spoilage and pathogenic bacteria. The aims of this study were: (i) to evaluate the influence of Lactobacillus paraplantarum FT259 on biofilm formation by Listeria monocytogenes by using culture method and fluorescence in situ hybridization (FISH) and, (ii) to partially characterize the bacteriocin produced by this strain isolated from Brazilian semi-hard cheese by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). We also performed polymerase chain reactions with primers for plantaricin NC8, plantaricin S and plantaricin W structural genes followed by DNA sequencing to possibly identify the bacteriocin produced. Results of microscopy assays demonstrated the complex and heterogenous structure of the biofilm formed by co-culture of both strains. SDS-PAGE analysis indicated that the partially purified bacteriocin presented a molecular mass around 3900 Da and, PCR-DNA sequencing detected the plantaricin NC8 gene. To our knowledge, this is the first report on the inhibitory effect of an L. paraplantarum strain on L monocytogenes biofilms, as well as the formation of biofilm by this LAB species. In addition, we have demonstrated L paraplantarum FT259 harbored the gene for plantaricin NC8 production, which may be responsible by the antimicrobial activity observed. (C) 2015 Elsevier Ltd. All rights reserved.