Gonadotropin-sensitive progesterone production by rhesus monkey luteal cells in vitro: a function of age of the corpus luteum during the menstrual cycle.
Gonadotropin-sensitive progesterone production by rhesus monkey luteal cells in vitro: a function of age of the corpus luteum during the menstrual cycle.
复制标题
恒河猴黄体细胞在体外产生促性腺激素敏感的黄体酮：月经周期期间黄体年龄的函数。
作者：
R. Stouffer;W. E. Nixon;B. Gulyas;G. Hodgen
在体外，在存在和不存在外源性促性腺激素的情况下，孕激素的生产进行了检查，在黄体细胞的悬浮液中，分离的恒河猴黄体在月经周期的各个阶段的胶原酶消化。在黄体中期（第15-19天）的周期中分离的细胞分泌孕酮长达6小时在体外。黄体中期细胞对生理浓度的人绒毛膜促性腺激素（hCG）有反应，低至0.1 ng hCG/ml时孕酮的产生显著增强（P <0.05）。用100 ng hCG/ml获得最大刺激。猕猴绒毛膜促性腺激素（mCG）和人促黄体生成素（hLH）均显著（P <0.01）增加孕酮的产生，而人促卵泡激素（hFSH）则无此作用。在对照条件下，在仅存在营养培养基（无外源性促性腺激素）的情况下，黄体中期细胞的孕酮合成活性显著（P <0.01）大于黄体晚期（周期的第22-28天）的细胞。此外，黄体中期细胞的孕酮生产一贯刺激（P小于0.01）的存在下，100 ng hCG/ml，而黄体晚期细胞不太敏感或无反应的外源性促性腺激素。离体黄体细胞孕酮合成活性与黄体湿重（r = 0.82，P <0.01）和术前外周血孕酮浓度（r = 0.66，P <0.01）呈正相关。这些结果表明，新鲜分离的黄体细胞反映了体内黄体的功能能力。可见，非生育期恒河猴黄体的年龄是影响黄体细胞孕酮合成能力和黄体细胞对促性腺激素反应性的重要因素。
Progesterone production in vitro, in the presence and absence of exogenous gonadotropin, was examined in suspensions of luteal cells, isolated by collagenase digestion of rhesus monkeys corpus luteum at various stages of the menstrual cycle. Cells isolated during mid-luteal phase (days 15-19) of the cycle secreted progesterone for up to 6 h in vitro. Mid-luteal phase cells were responsive to physiologic concentrations of human chorionic gonadotropin (hCG), with progesterone production significantly (P less than 0.05) enhanced by as little as 0.1 ng hCG/ml. Maximal stimulation was obtained with 100 ng hCG/ml. Both macaque chorionic gonadotropin (mCG) and human luteinizing hormone (hLH) significantly (P less than 0.01) increased progesterone production, while human follicle stimulating hormone (hFSH) did not. Under control conditions, in the presence of nutrient medium alone (no exogenous gonadotropin), the progesterone synthetic activity of mid-luteal phase cells was significantly (P less than 0.01) greater than that of cells from late luteal phase (days 22-28) of the cycle. Moreover, progesterone production by mid-luteal phase cells was consistently stimulated (P less than 0.01) by the presence of 100 ng hCG/ml, whereas late luteal phase cells were less sensitive or unresponsive to exogenous gonadotropin. The progesterone synthetic activity of luteal cells in vitro correlated positively with both the wet weight of the excised corpus luteum (r = 0.82, P less than 0.01) and the peripheral serum progesterone concentration immediately preceding luteectomy (r = 0.66, P less than 0.01). These findings suggest that freshly isolated luteal cells reflect the functional capability of the corpus luteum in vivo. It is apparent that the age of the rhesus monkey corpus luteum of the non-fertile menstrual cycle is an important factor governing luteal cell progesterone synthetic capability and luteal cell responsiveness to gonadotropin in vitro.