Energy expenditure and body composition of chronically maintained decerebrate rats in the fed and fasted condition
Energy expenditure and body composition of chronically maintained decerebrate rats in the fed and fasted condition
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DOI：
10.1210/en.2005-1156
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发表时间：
2006-03-01
期刊：
影响因子：
4.8
通讯作者：
Grill, HJ
中科院分区：
文献类型：
--
作者：
Harris, RBS;Kelso, EW;Grill, HJ
尾侧脑干的贡献，以适应饥饿进行了测试，长期保持去大脑（CD）和神经系统完整的控制。所有大鼠均灌胃喂食一定量的饲料，以维持对照组的体重增加。对CD大鼠进行两阶段手术，以在间脑交界处产生完全横断的神经轴。1周后，将大鼠置于间接热量计中，连续4d测定24 h能量消耗。然后将CD组和对照组各一半饥饿48小时。与对照组相比，喂食CD的大鼠保持较低的体温（35 ℃），每单位无脂肪质量的能量消耗相似，但呼吸商升高。与对照组相比，他们体重增加较少，瘦组织减少20%，脂肪增加60%。循环瘦素、脂联素和胰岛素升高，血糖正常，但睾酮显著降低。对饥饿的反应在CD和对照组中相似;它们减少能量消耗，降低呼吸商，表明脂质利用，保卫体温，动员脂肪，降低血清瘦素和胰岛素，调节血糖。这些数据清楚地表明，孤立的尾脑干是足以调解许多方面的能量反应饥饿。在完整的动物中，这些反应可以通过更多的吻侧脑区的贡献或通过前脑和后脑之间的交流来改善。在前脑缺乏交流的情况下，尾脑干不足以维持进食或禁食动物的睾酮水平或瘦肉组织。
The contribution of the caudal brainstem to adaptation to starvation was tested using chronically maintained decerebrate (CD) and neurologically intact controls. All rats were gavage fed an amount of diet that maintained weight gain in controls. CD rats were subjected to a two-stage surgery to produce a complete transection of the neuroaxis at the mesodiencephalic juncture. One week later, the rats were housed in an indirect calorimeter, and 24 h energy expenditure was measured for 4d. One half of each of the CD and control groups was then starved for 48 h. Fed CD rats maintained a lower body temperature (35 C), a similar energy expenditure per unit fat-free mass but an elevated respiratory quotient compared with controls. They gained less weight, had 20% less lean tissue, and had 60% more fat than controls. Circulating leptin, adiponectin, and insulin were elevated, glucose was normal, but testosterone was dramatically reduced. Responses to starvation were similar in CD and controls; they reduced energy expenditure, decreased respiratory quotient, indicating lipid utilization, defended body temperature, mobilized fat, decreased serum leptin and insulin, and regulated plasma glucose. These data clearly demonstrate that the isolated caudal brainstem is sufficient to mediate many aspects of the energetic response to starvation. In intact animals, these responses may be refined by a contribution by more rostral brain areas or by communication between fore- and hind-brain. In the absence of communication from the fore-brain, the caudal brainstem is inadequate for maintenance of testosterone levels or lean tissue in fed or fasted animals.