Multiplex analysis of cytokines in the serum and cerebrospinal fluid of patients with Alzheimer's disease by color-coded bead technology.
Multiplex analysis of cytokines in the serum and cerebrospinal fluid of patients with Alzheimer's disease by color-coded bead technology.
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DOI：
10.3988/jcn.2008.4.2.84
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发表时间：
2008-06
期刊：
影响因子：
--
通讯作者：
Kang SW
中科院分区：
文献类型：
--
作者：
Choi C;Jeong JH;Jang JS;Choi K;Lee J;Kwon J;Choi KG;Lee JS;Kang SW
神经退行性疾病的有效治疗的可用性和前景正在增加早期诊断的重要性。拥有阿尔茨海默病（AD）的分子和生化标志物将补充临床方法，并进一步实现早期和准确诊断的目标。在评估中组合多种生物标志物显著增加了生化测试的灵敏度和特异性。在这项研究中，我们使用基于颜色编码珠的Luminex技术来测试使用趋化因子和细胞因子作为AD生化标志物的潜力。我们测量了32例初治患者（13例对照，11例AD和8例帕金森病[PD]）血清和脑脊液（CSF）中22种趋化因子和细胞因子的水平。MCP-1是CSF中唯一可检测到的细胞因子，其水平在对照组和疾病组之间没有差异。然而，血清嗜酸性粒细胞趋化因子浓度显着高于AD患者比对照组。多种炎症介质的分析显示，在AD和PD的鉴别诊断中，其CSF和血清浓度存在微小差异。这些结果提供了证据，免疫反应不是AD和PD发病机制的主要贡献者。
The availability and promise of effective treatments for neurodegenerative disorders are increasing the importance of early diagnosis. Having molecular and biochemical markers of Alzheimer's disease (AD) would complement clinical approaches, and further the goals of early and accurate diagnosis. Combining multiple biomarkers in evaluations significantly increases the sensitivity and specificity of the biochemical tests. In this study, we used color-coded bead-based Luminex technology to test the potential of using chemokines and cytokines as biochemical markers of AD. We measured the levels of 22 chemokines and cytokines in the serum and cerebrospinal fluid (CSF) of 32 de novo patients (13 controls, 11 AD, and 8 Parkinson's disease [PD]). MCP-1 was the only cytokine detectable in CSF, and its levels did not differ between control and disease groups. However, the serum concentration of eotaxin was significantly higher in AD patients than in the control group. The analysis of multiple inflammatory mediators revealed marginal differences in their CSF and serum concentrations for the differential diagnosis of AD and PD. These results provide evidence that immunological responses are not major contributors to the pathogenesis of AD and PD.
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影响因子：
8.6
作者：
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通讯作者：
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