Encapsulation, permeability, and cellular uptake characteristics of hollow nanometer-sized conductive polymer capsules
Encapsulation, permeability, and cellular uptake characteristics of hollow nanometer-sized conductive polymer capsules
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DOI：
10.1021/jp010820d
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发表时间：
2001-09-20
影响因子：
3.3
通讯作者：
Feldheim, DL
中科院分区：
文献类型：
--
作者：
Marinakos, SM;Anderson, MF;Feldheim, DL
介绍了以纳米金粉为模板合成中空聚吡咯、聚N-甲基吡咯和聚3-甲基噻吩型聚合物的方法。小分子在胶囊壳中的扩散系数随聚合物、聚合物氧化状态和聚合物合成过程中加入的反阴离子的不同而变化近3个数量级。在聚合和粒子蚀刻之前，通过将小分子(蒽醌)和酶(辣根过氧化物酶)附着到模板粒子上，将它们捕获在中空胶囊中。在干净的甲苯中，一层薄薄的聚吡咯壳对酶的保护作用是未包封的酶的2倍。最后，通过将Partice悬浮液应用于3T3小鼠成纤维细胞，检测了将导电聚合物纳米颗粒用于细胞内递送或诊断的可能性。大小从25到100 nm的颗粒可以被成纤维细胞吞噬，而不会影响细胞的活力。
The use of nanometer-sized gold particles as templates for the synthesis of hollow poly(pyrrole), poly(N-methylpyrrole), and poly(3-methylthiophene) is described in this paper. Diffusion coefficients of small molecules through the capsule shell were found to vary by almost 3 orders of magnitude depending on the polymer, polymer oxidation state, and counteranion incorporated during polymer synthesis. A small molecule (anthraquinone) and an enzyme (horseradish peroxidase) were trapped inside hollow capsules by attaching them to the template particle prior to polymerization and particle etching. A thin poly(pyrrole) shell protected the enzyme 2 times longer in neat toluene compared to unencapsulated enzyme. Finally, the potential for using conductive polymer nanoparticles for intracellular delivery or diagnostics was examined by administering partice suspensions to 3T3 murine fibroblasts. Particles ranging in size from 25 to 100 nm were engulfed by fibroblasts without compromising cell viability.