Epigenetic processes in plant stress priming: Open questions and new approaches.
Epigenetic processes in plant stress priming: Open questions and new approaches.
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DOI：
10.1016/j.pbi.2023.102432
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发表时间：
2023-07
影响因子：
9.5
通讯作者：
C. Harris;A. Amtmann;J. Ton
中科院分区：
文献类型：
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作者：
C. Harris;A. Amtmann;J. Ton
启动反应反映了植物对环境胁迫的记忆能力，并提高了它们对反复出现的胁迫的反应。DNA和相关组蛋白的表观遗传修饰可能在同一植物中携带短期和长期记忆或介导跨代效应，但证据在很大程度上仍然是间接的。新的实验工具现在使科学家能够进行有针对性的操作，防止或在基因组的特定位置产生特定的表观遗传修饰。这种“反向表观遗传学”方法允许询问个体引发诱导的修饰与它们在反复胁迫下改变基因表达和植物性能的作用之间的因果关系。此外，将定点表观遗传操作与条件和细胞类型特异性启动子相结合，创造了测试和工程师引发时空模式的新机会。
Priming reflects the capacity of plants to memorise environmental stress experience and improve their response to recurring stress. Epigenetic modifications in DNA and associated histone proteins may carry short-term and long-term memory in the same plant or mediate transgenerational effects, but the evidence is still largely circumstantial. New experimental tools now enable scientists to perform targeted manipulations that either prevent or generate a particular epigenetic modification in a particular location of the genome. Such ‘reverse epigenetics’ approaches allow for the interrogation of causality between individual priming-induced modifications and their role for altering gene expression and plant performance under recurring stress. Furthermore, combining site-directed epigenetic manipulation with conditional and cell-type specific promoters creates novel opportunities to test and engineer spatiotemporal patterns of priming.