Transcriptome and hyperspectral profiling allows assessment of phosphorus nutrient status in rice under field conditions
Transcriptome and hyperspectral profiling allows assessment of phosphorus nutrient status in rice under field conditions
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DOI：
10.1111/pce.14280
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发表时间：
2022-02-25
影响因子：
7.3
通讯作者：
Sato, Yutaka
中科院分区：
文献类型：
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作者：
Takehisa, Hinako;Ando, Fuminori;Sato, Yutaka
磷（P）是作物生产不可或缺的常量营养素之一，因此了解植物适应低磷条件的潜力非常重要。我们比较了四个水稻品种在不同速效磷含量的两个田间生长过程中的生长和叶片全基因组转录组，并从外观性状和内部磷营养状况（通过分析磷指示基因的表达谱来测量）进一步分析了正常生长的可接受的磷水平范围。这表明水稻植物对中度缺磷条件具有稳健性，表达了磷获取和利用系统，而不对产量潜力产生任何影响，并且磷指示基因表达可能是植物磷状态早期诊断的有用指标。为了开发一种简单的 P 状态评估方法，我们尝试使用反射光谱和高光谱成像来预测表达水平，从而提供具有良好性能的模型。我们的研究结果表明，水稻植株具有适应田间适度低磷条件的潜力，并表明高光谱技术是简单测量反映内部营养条件的分子水平动态的有用工具之一。
Phosphorus (P) is one of the macronutrients indispensable for crop production, and therefore it is important to understand the potential of plants to adapt to low P conditions. We compared growth and leaf genome-wide transcriptome of four rice cultivars during growth between two fields with different amount of available phosphate and further analysed the acceptable range of P levels for normal growth from the view of both appearance traits and internal P nutrient status, which was measured by profiling the expression of the P indicator gene. This demonstrated that rice plants have a robustness to moderate P-deficient conditions expressing a system for P acquisition and usage without any effects on yield potential and that P indicator gene expression could be a useful index for early diagnosis of P status in plants. To develop a simple method for assessment of P status, we tried to predict the expression level using reflectance spectroscopy and hyperspectral imaging, thereby providing models with good performance. Our findings suggest that rice plants have the potential to adapt to moderate low P conditions in the field and showed that the hyperspectral technique is one of the useful tools for simple measurement of molecular-level dynamics reflecting internal nutrient conditions.