Niche Differentiation of Comammox Nitrospira in the Mudflat and Reclaimed Agricultural Soils Along the North Branch of Yangtze River Estuary.
Niche Differentiation of Comammox Nitrospira in the Mudflat and Reclaimed Agricultural Soils Along the North Branch of Yangtze River Estuary.
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长江口北支滩涂及复垦农田土壤中Comammox Nitrospira的生态位分化
DOI：
10.3389/fmicb.2020.618287
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发表时间：
2020
影响因子：
5.2
通讯作者：
Wang B
中科院分区：
文献类型：
--
作者：
Wang X;Lu L;Zhou X;Tang X;Kuang L;Chen J;Shan J;Lu H;Qin H;Adams J;Wang B
完全氨氧化（comammox）的发现，在单个生物体内通过亚硝酸盐将氨氧化为硝酸盐，重新定义了由两个官能基团（氨氧化和亚硝酸盐氧化微生物）驱动的两步硝化的传统认识。然而，对河口滩涂及其开垦的农业土壤中氮螺旋藻的分布和生态位分异的认识仍然有限。本文研究了长江口北部泥滩和垦殖农业土壤中氮螺旋藻的丰度、多样性和群落结构。定量PCR结果显示，comammox的amoA基因丰度在几乎所有样品中都低于氨氧化菌（AOB）。amoA基因扩增子测序结果显示，原泥滩与复垦农用土壤间的群落结构存在显著差异（P < 0.001），表明群落生态位存在差异（A.1、A.2和B）。在泥滩中以a - 1支系为主，在农业土壤中以B支系为主。然而，A.2分支的成员可以清楚地分为两个类群，即泥滩偏好类群和农业土壤偏好类群，这表明该亚分支的生态偏好更为复杂。此外，盐度、有机质（OM）和NO3——N对河口环境中硝化梭菌的分布有显著影响。进化支A.1和近半数进化支A.2成员与盐度呈正相关，与OM和NO3—N浓度负相关。与此相反，B支系和a支系2的另一半成员则表现出完全相反的模式：与盐度负相关，与OM和NO3—N正相关。共现网络表明，同一（亚）支系内的操作分类单位（OTUs）多为正相关，表明同一（亚）支系成员间具有相似的生态位偏好。综上所述，我们的研究结果揭示了河口生态系统中氮螺旋藻的生态位分化，盐度和有机质是造成其生态分布格局差异的主要因素。
The discovery of complete ammonia oxidation (comammox), oxidizing ammonia to nitrate via nitrite in a single organism, has redefined the traditional recognition of the two-step nitrification driven by two functional groups (ammonia-oxidizing and nitrite-oxidizing microorganisms). However, the understanding of the distribution and niche differentiation of comammox Nitrospira in the estuarine mudflats and their reclaimed agricultural soils is still limited. Here, we investigated the abundance, diversity and community structures of comammox Nitrospira in the mudflats and the reclaimed agricultural soils in the northern Yangtze River estuary. Quantitative PCR showed the abundances of amoA genes of comammox were lower than that of ammonia-oxidizing bacteria (AOB) in nearly all samples. Amplicon sequencing of amoA genes revealed that the community structures of comammox Nitrospira were significantly (P < 0.001) different between the original mudflats and the reclaimed agricultural soils, indicating niche differentiation among comammox Nitrospira clades (clade A.1, clade A.2, and clade B). The clade A.1 was the dominant group of comammox Nitrospira in the mudflats, while clade B predominated in the agricultural soils. However, the members of clade A.2 could be clearly divided into two groups, the mudflat-preferred and agricultural soil-preferred groups, suggesting more complicated ecological preferences within this sub-clade. Furthermore, it was demonstrated that salinity, organic matter (OM) and NO3–-N had a significantly influence on the distribution of comammox Nitrospira in the estuarine environment. Clade A.1 and nearly half members of clade A.2 were positively correlated with salinity, and negatively correlated with the concentrations of OM and NO3–-N. In contrast, the clade B and the other half members of clade A.2 showed the exact opposite pattern: a negative correlation with salinity and positive correlation with OM and NO3–-N. The co-occurrence network demonstrated that the operational taxonomic units (OTUs) within the same (sub-)clade were mostly positively correlated, indicating the similar niche preferences among the members from the same (sub-)clade of comammox Nitrospira. Taken together, our results revealed the niche differentiation of comammox Nitrospira in estuarine ecosystems where salinity and OM were the primary factors responsible for the distinct ecological distribution patterns.
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影响因子：
2.7
作者：
Benjamini, Yoav;Krieger, Abba M.;Yekutieli, Daniel
通讯作者：
Yekutieli, Daniel
影响因子：
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作者：
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通讯作者：
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