SIMIAN VIRUS 40 DNA SEQUENCES IN DNA OF HEALTHY ADULT MICE DERIVED FROM PREIMPLANTATION BLASTOCYSTS INJECTED WITH VIRAL DNA
SIMIAN VIRUS 40 DNA SEQUENCES IN DNA OF HEALTHY ADULT MICE DERIVED FROM PREIMPLANTATION BLASTOCYSTS INJECTED WITH VIRAL DNA
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DOI：
10.1073/pnas.71.4.1250
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
MINTZ, B
中科院分区：
文献类型：
--
作者：
JAENISCH, R;MINTZ, B
用猴病毒40 （SV40）病毒DNA在小鼠胚腔内微注射。手术移植到假孕代孕母亲的子宫后，大约40%的囊胚发育到足月，并在1岁时成为没有明显肿瘤的健康成人。对这些动物各器官提取的DNA进行了sv40特异性DNA序列的分子杂交试验。在大约40%的成年幸存者的某些器官中，每二倍体小鼠DNA值在0.5到13个SV40基因组当量之间；这表明每个胚胎的注射量有了实质性的增加。结果与SV40 DNA可能已整合到宿主基因组的工作假设一致；或者，病毒DNA可能作为染色体外实体或通过裂解感染在少数允许细胞中复制。因此，病毒DNA从植入前阶段到成年期的持续存在可能为肿瘤病毒垂直传播和表达的实验研究提供新的工具。
Explanted mouse blastocysts were microinjected in the blastocoel cavity with simian virus 40 (SV40) viral DNA. After surgical transfer to the uteri of pseudopregnant surrogate mothers, approximately 40% of the blastocysts developed to term and became healthy adults without apparent tumors at 1 year of age. Molecular hybridization tests for the presence of SV40-specific DNA sequences were conducted on DNA extracted from various organs of these animals. Between 0.5 and 13 SV40 genome equivalents per diploid mouse DNA value were found in some organs of approximately 40% of the adult survivors; this represents a substantial augmentation of the amount administered per embryo. The results are consistent with the working hypothesis that the SV40 DNA may have been integrated into the host genome; alternatively, the viral DNA may have replicated as an extrachromosomal entity or by lytic infection in a few permissive cells. Persistence of the viral DNA from preimplantation stages to adult life may thus provide a new tool for experimental investigation of vertical transmission and expression of tumor viruses.