Extending and Running the Mosquito Small RNA Genomics Resource Pipeline.
Extending and Running the Mosquito Small RNA Genomics Resource Pipeline.
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扩展和运行 Mosquito 小 RNA 基因组学资源管道。
DOI：
10.1007/978-1-0716-2380-0_20
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Lau,NelsonC
中科院分区：
文献类型：
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作者：
Dayama,Gargi;Bulekova,Katia;Lau,NelsonC
蚊子小RNA基因组学(MSRG)资源是对蚊子细胞培养物以及体细胞和性腺组织的小RNA转录本进行分析的资源库。这一资源允许比较不同蚊种转座子和病毒产生的小RNA的调控动态。本章介绍了将MSRG资源管道设置为新安装的程序，详细介绍了基因组参考和注释文件的必要集合以及microRNAs(MiRNAs)发夹序列、转座子重复序列和病毒基因组序列的列表。正确执行MSRG资源管道产生的输出适合生物学家用桌面和电子表格软件进一步分析，以深入了解节肢动物内源小RNA种群与病毒衍生小RNA之间的平衡，其中包括Piwi相互作用RNA(PiRNAs)和内源小干扰RNAs(SiRNAs)。
The Mosquito Small RNA Genomics (MSRG) resource is a repository of analyses on the small RNA transcriptomes of mosquito cell cultures and somatic and gonadal tissues. This resource allows for comparing the regulation dynamics of small RNAs generated from transposons and viruses across mosquito species. This chapter covers the procedures to set up the MSRG resource pipeline as a new installation by detailing the necessary collection of genome reference and annotation files and lists of microRNAs (miRNAs) hairpin sequences, transposon repeats consensus sequences, and virus genome sequences. Proper execution of the MSRG resource pipeline yields outputs amenable to biologists to further analyze with desktop and spreadsheet software to gain insights into the balance between arthropod endogenous small RNA populations and the proportions of virus-derived small RNAs that include Piwi-interacting RNAs (piRNAs) and endogenous small interfering RNAs (siRNAs).