Host response to mycobacterial infection in the alcoholic rat: Male and female dimorphism
Host response to mycobacterial infection in the alcoholic rat: Male and female dimorphism
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DOI：
10.1016/s0741-8329(98)00004-4
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发表时间：
1998-10-01
期刊：
影响因子：
2.3
通讯作者：
Gartside, P
中科院分区：
文献类型：
--
作者：
Li, X;Grossman, CJ;Gartside, P
酗酒者对结核病的易感性增加。易感性改变的一种解释是 T 淋巴细胞调节的变化。为了评估这一点，24 只雄性和 24 只雌性 Sprague-Dawley 大鼠接受了利伯型液体乙醇饮食 (LED) 或等热量对照 (LCD) 治疗。 2周后，一半受试者感染BCG（10（8）个集落形成单位）并在42天后处死。通过流式细胞术对脾辅助细胞 (CD4) 和抑制/细胞毒性 (CD8) 细胞进行定量。通过三向方差分析，脾细胞结构因感染而显着增加 (p < 0.0001)，但因 LED 而受到抑制 (p = 0.0002)。存在边际性别差异 (p = 0.065)，女性饮酒时的反应降低 35%。检查淋巴细胞亚群，在感染（BCG）和酒精治疗（LED）后观察到最显着的变化。 CD4 水平因 LED 降低 (p = 0.0002)，但因感染而显着升高 (p < 0.0001)，产生高度显着的相互作用，影响绝对数量 (p < 0.0001) 和相对百分比 (p = 0.0078)。 CD8 仅受感染影响 (p < 0.0001)。这导致感染相关的 CD4/CD8 比率增加，而 LED 则较低 (p = 0.0032)。脾 T 淋巴细胞（主要是 CD4）参与宿主对 BCG 肝炎的反应，并受到 LED 的不利影响，这可能导致易感性增加。由爱思唯尔科学公司出版
Increased susceptibility to tuberculosis occurs in the alcoholic. One explanation for the altered susceptibility is a change in T-lymphocyte modulation. To evaluate this, 24 male and 24 female Sprague-Dawley rats were treated with either a Lieber-type liquid ethanol diet (LED) or an isocaloric control (LCD). After 2 weeks, half the subjects were infected with BCG (10(8) colony-forming units) and sacrificed after 42 days. Splenic helper (CD4) and suppressor/cytoxic (CD8) cells were quantitated by flow cytometry. By three-way analysis of variance, splenic cellularity was significantly increased by infection (p < 0.0001) but suppressed by LED (p = 0.0002). There was a marginal sexual difference (p = 0.065) with females exhibiting a 35% lower response while on alcohol. Examining lymphocyte subsets, the most significant changes were observed after infection (BCG) and alcohol treatment (LED). CD4 levels were deminished by LED (p = 0.0002) but markedly increased by infection (p < 0.0001), producing a highly significant interaction that affected both absolute number (p < 0.0001) and relative percent present (p = 0.0078). CD8 was influenced only by infection (p < 0.0001). This resulted in a infection-related increase in the CD4/CD8 ratio which was lower with LED (p = 0.0032). Splenic T-lymphocytes, predominately CD4, are involved in the host response to BCG hepatitis and are adversely influenced by LED, which may contribute to increased susceptibility. Published by Elsevier Science Inc.