The Effect of Serum Origin on Tissue Engineered Skeletal Muscle Function
The Effect of Serum Origin on Tissue Engineered Skeletal Muscle Function
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DOI：
10.1002/jcb.24938
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发表时间：
2014-12-01
影响因子：
4
通讯作者：
Baar，Keith
中科院分区：
文献类型：
--
作者：
Khodabukus，Alastair;Baar，Keith
骨骼肌表型受遗传、激素和电输入之间复杂的相互作用调节。然而，由于这些因素在体内的相互关系，很难确定一个比另一个重要。在过去的5年中，我们在欧盟（EU）和美国（US）使用相同的C2C12细胞克隆来设计骨骼肌。引人注目的是，肌肉收缩的动力是截然不同的。因此，在这项研究中，我们试图确定激素环境（胎牛血清（FBS）的来源）是否可以改变工程肌肉表型。在美国原产时间-峰值张力（2.2倍）、半松弛（2.6倍）和抗疲劳性（提高25%）的血清中工程肌肉均显示出向较慢表型转变的迹象。尽管收缩速率发生了戏剧性的变化，但肌凝蛋白重链的表达是相同的。相反，收缩速度与钙释放/敏感蛋白（DHPR =低3.1倍，慢速CSQ =高3.4倍，慢速TnT =高2.4倍）和钙摄取蛋白（慢速SERCA =高1.7倍，小白蛋白=低41倍）的变化有关。钙动力学的这些变化伴随着代谢酶的部分变化，但不能用所谓的肌肉表型调节因子来解释。这些数据表明，美国农业部和欧盟来源的血清激素差异导致钙处理的转变，从而导致工程肌肉功能的巨大变化。j .细胞。中国生物医学工程学报，2014,31(2):444 - 444。©2014 Wiley期刊公司
Skeletal muscle phenotype is regulated by a complex interaction between genetic, hormonal, and electrical inputs. However, because of the interrelatedness of these factors in vivo it is difficult to determine the importance of one over the other. Over the last 5 years, we have engineered skeletal muscles in the European Union (EU) and the United States (US) using the same clone of C2C12 cells. Strikingly, the dynamics of contraction of the muscles was dramatically different. Therefore, in this study we sought to determine whether the hormonal milieu (source of fetal bovine serum (FBS)) could alter engineered muscle phenotype. In muscles engineered in serum of US origin time‐to‐peak tension (2.2‐fold), half relaxation (2.6‐fold), and fatigue resistance (improved 25%) all showed indications of a shift towards a slower phenotype. Even though there was a dramatic shift in the rate of contraction, myosin heavy chain expression was the same. The contraction speed was instead related to a shift in calcium release/sensitivity proteins (DHPR = 3.1‐fold lower, slow CSQ = 3.4‐fold higher, and slow TnT = 2.4‐fold higher) and calcium uptake proteins (slow SERCA = 1.7‐fold higher and parvalbumin = 41‐fold lower). These shifts in calcium dynamics were accompanied by a partial shift in metabolic enzymes, but could not be explained by purported regulators of muscle phenotype. These data suggest that hormonal differences in serum of USDA and EU origin cause a shift in calcium handling resulting in a dramatic change in engineered muscle function. J. Cell. Biochem. 115: 2198–2207, 2014. © 2014 Wiley Periodicals, Inc.
鸡肌肉培养物中肌原纤维积累的调节：钙和溶酶体参与收缩蛋白不均匀周转的证据。
影响因子：
7.8
作者：
Silver, G;Etlinger, J D
通讯作者：
Etlinger, J D