Superparamagnetic iron oxide-encapsulating polymersome nanocarriers for biofilm eradication
Superparamagnetic iron oxide-encapsulating polymersome nanocarriers for biofilm eradication
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DOI：
10.1016/j.biomaterials.2016.12.011
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发表时间：
2017-03-01
期刊：
影响因子：
14
通讯作者：
Webster, Thomas J.
中科院分区：
文献类型：
--
作者：
Geilich, Benjamin M.;Gelfat, Ilia;Webster, Thomas J.
耐药性生物膜感染的流行率和严重程度不断上升，对全球公共卫生构成了令人担忧的威胁。在这里，合成了生物相容性多室纳米载体，含有疏水性超顺磁性氧化铁纳米颗粒（SPION）和亲水性抗生素甲氧西林，用于治疗医疗器械相关感染。发现SPION共封装赋予独特的性质，与单个SPION相比，增强了纳米载体弛豫率和磁性。这些氧化铁封装聚合物囊泡（IOPs）穿透20 μ m厚的表皮葡萄球菌生物膜与高效率的应用程序的外部磁场。三维激光扫描共聚焦显微镜显示差异细菌死亡作为药物和SPION负载的函数。使用含有40 μ g/mL SPION和20 μ g/mL甲氧西林的优化IOP制剂实现了整个生物膜厚度中所有细菌的完全根除。重要的是，该制剂对耐甲氧西林生物膜细胞具有选择性毒性，但对哺乳动物细胞没有毒性。这些新的铁氧化物封装聚合物囊泡表明，它是可能的，以克服耐药的生物膜，通过控制含有两种或更多种治疗剂的纳米载体的定位。(C)2016爱思唯尔有限公司版权所有
The rising prevalence and severity of antibiotic-resistant biofilm infections poses an alarming threat to public health worldwide. Here, biocompatible multi-compartment nanocarriers were synthesized to contain both hydrophobic superparamagnetic iron oxide nanoparticles (SPIONs) and the hydrophilic antibiotic methicillin for the treatment of medical device-associated infections. SPION co-encapsulation was found to confer unique properties, enhancing both nanocarrier relaxivity and magneticity compared to individual SPIONs. These iron oxide-encapsulating polymersomes (IOPs) penetrated 20 mu m thick Staphylococcus epidermidis biofilms with high efficiency following the application of an external magnetic field. Three-dimensional laser scanning confocal microscopy revealed differential bacteria death as a function of drug and SPION loading. Complete eradication of all bacteria throughout the biofilm thickness was achieved using an optimized IOP formulation containing 40 mu g/mL SPION and 20 mu g/mL of methicillin. Importantly, this formulation was selectively toxic towards methicillin-resistant biofilm cells but not towards mammalian cells. These novel iron oxide-encapsulating polymersomes demonstrate that it is possible to overcome antibiotic-resistant biofilms by controlling the positioning of nanocarriers containing two or more therapeutics. (C) 2016 Elsevier Ltd. All rights reserved.