Detection of p53 and Bcl-2 expression in cutaneous hemangioma through the quantum dot technique.
Detection of p53 and Bcl-2 expression in cutaneous hemangioma through the quantum dot technique.
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量子点技术检测皮肤血管瘤中p53和Bcl-2的表达
DOI：
10.3892/ol.2017.5856
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发表时间：
2017-05
期刊：
影响因子：
2.9
通讯作者：
Zhang DL
中科院分区：
文献类型：
--
作者：
Tang T;Zhang DL
血管瘤是婴幼儿血管良性肿瘤中最常见的类型之一。本研究旨在探讨B细胞淋巴瘤2（Bcl-2）和肿瘤蛋白p53（p53）在血管瘤细胞增殖和凋亡中的作用。2007年1月至2010年12月期间，从武汉大学人民医院病理科（中国武汉）共获得38例石蜡包埋的血管瘤标本（16例男性和22例女性）和另外5例石蜡包埋的健康周围组织标本。采用免疫组化、苏木精-伊红染色、量子点双染法检测血管瘤及周围正常皮肤组织中增殖细胞核抗原（PCNA）、Bcl-2和p53的表达。根据Mulliken标准和PCNA表达情况将所有血管瘤标本分为增殖期和消退期。使用多光谱成像系统分析量子点双重染色的结果。采用单因素方差分析和Student-Newman-Keuls q检验对数据进行统计学分析。其中增生期24例，消退期14例。免疫组化结果显示，Bcl-2和p53在增生期血管瘤组织中呈高表达，在消退期血管瘤和健康组织中呈低表达。量子点双染结果统计学分析显示，增生期血管瘤中Bcl-2和p53的表达明显高于消退期（P<0.05）和正常组织（P<0.05）。Bcl-2和p53的表达在消退中的血管瘤和周围健康组织样本之间没有显著差异。Bcl-2和p53在增殖期血管瘤中的高表达提示Bcl-2可能通过抑制内皮细胞凋亡而导致内皮细胞增殖与凋亡的失衡。此外，p53还可能促进血管瘤内皮细胞的增殖。
Hemangioma is one of the most common types of infantile vascular benign tumor. The aim of the present study was to investigate the role of B-cell lymphoma 2 (Bcl-2) and tumor protein p53 (p53) in the proliferation and apoptosis of hemangioma cells. A total of 38 paraffin-embedded hemangioma specimens (16 males and 22 females) and another 5 paraffin-embedded healthy surrounding tissue samples, collected between January 2007 and December 2010, were obtained from the Department of Pathology at Renmin Hospital of Wuhan University (Wuhan, China). Immunohistochemistry, hematoxylin and eosin staining, and quantum dot double staining were used to detect the expression of proliferating cell nuclear antigen (PCNA), Bcl-2 and p53 in hemangioma and healthy surrounding skin tissue samples. All hemangioma specimens were classified into proliferative or the involuting stage hemangioma according to Mulliken's criteria and their expression of PCNA. The results of the quantum dot double staining were analyzed using a multi-spectral imaging system. One-way analysis of the variance and the Student-Newman-Keuls q test were performed to statistically analyze the data. There were 24 cases of proliferative stage and 14 cases of involuting stage hemangioma among the specimens. Immunohistochemical analysis results indicated a high expression of Bcl-2 and p53 in proliferative stage hemangioma tissue samples, and low expression in involuting stage hemangioma and healthy tissue samples. Statistical analysis of the results from quantum dot double staining demonstrated that the expression of Bcl-2 and p53 in proliferative hemangioma was significantly increased compared with that in involuting stage specimens (P<0.05) and healthy tissue samples (P<0.05). No significant difference in Bcl-2 and p53 expression was identified between the involuting hemangioma and healthy surrounding tissue samples. The higher expression of Bcl-2 and p53 in proliferative hemangioma suggests that Bcl-2 may cause an imbalance between endothelial cell proliferation and apoptosis through the inhibition of endothelial cell apoptosis. Furthermore, p53 may promote the proliferation of endothelial cells in proliferative hemangioma.