Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS)
Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS)
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DOI：
10.1016/j.aca.2012.04.009
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发表时间：
2012-10-31
影响因子：
6.2
通讯作者：
Ashley, David L.
中科院分区：
文献类型：
--
作者：
Alwis, K. Udeni;Blount, Benjamin C.;Ashley, David L.
挥发性有机化合物（VOC）在环境中无处不在，来自许多不同的自然和人为来源，包括烟草烟雾。长期接触某些挥发性有机化合物可能会增加患癌症、出生缺陷和神经认知障碍的风险。因此。挥发性有机化合物暴露是一个重要的公共卫生问题。尿液VOC代谢物是评估VOC暴露的有用生物标志物，因为与血液和呼吸中的VOC相比，尿液代谢物的非侵入性采样和更长的生理半衰期。我们开发了一种方法，使用反相超高效液相色谱（UPLC）电喷雾串联质谱（ESI/MSMS），同时定量28个尿挥发性有机化合物代谢物作为暴露的生物标志物。我们描述了一种方法，监测丙烯醛，丙烯酰胺，丙烯腈，苯，1-溴丙烷，1，3-丁二烯，二硫化碳，巴豆醛，氰化物，N，N-二甲基甲酰胺，N，N-二甲基甲酰胺，环氧乙烷，环氧丙烷，苯乙烯，四氯乙烯，甲苯，三氯乙烯，氯乙烯和二甲苯的代谢物。该方法具有准确度（加标基质的平均准确度范围为84 - 104%）、灵敏度（检测限范围为0.5 - 20 ng/mL）和精密度（相对标准偏差范围为2.5 - 11%）。我们将这种方法应用于从1203名非吸烟者和347名吸烟者收集的尿液样本，并证明与非吸烟者相比，吸烟者的烟草相关生物标志物水平显着升高。我们发现血清可替宁与大多数烟草相关生物标志物之间存在显著相关性（p < 0.0001）。这些发现证实，该方法可以有效地量化暴露于挥发性有机物的人群中的尿VOC代谢物。由爱思唯尔公司出版
Volatile organic compounds (VOCs) are ubiquitous in the environment, originating from many different natural and anthropogenic sources, including tobacco smoke. Long-term exposure to certain VOCs may increase the risk for cancer, birth defects, and neurocognitive impairment. Therefore. VOC exposure is an area of significant public health concern. Urinary VOC metabolites are useful biomarkers for assessing VOC exposure because of non-invasiveness of sampling and longer physiological half-lives of urinary metabolites compared with VOCs in blood and breath. We developed a method using reversed-phase ultra high performance liquid chromatography (UPLC) coupled with electrospray ionization tandem mass spectrometry (ESI/MSMS) to simultaneously quantify 28 urinary VOC metabolites as biomarkers of exposure. We describe a method that monitors metabolites of acrolein, acrylamide, acrylonitrile, benzene, 1-bromopropane, 1,3-butadiene, carbon-disulfide, crotonaldehyde, cyanide, N,N-dimethylformamide, ethylbenzene, ethylene oxide, propylene oxide, styrene, tetrachloroethylene, toluene, trichloroethylene, vinyl chloride and xylene. The method is accurate (mean accuracy for spiked matrix ranged from 84 to104%), sensitive (limit of detection ranged from 0.5 to 20 ng mL(-1)) and precise (the relative standard deviations ranged from 2.5 to 11%). We applied this method to urine samples collected from 1203 non-smokers and 347 smokers and demonstrated that smokers have significantly elevated levels of tobacco-related biomarkers compared to non-smokers. We found significant (p < 0.0001) correlations between serum cotinine and most of the tobacco-related biomarkers measured. These findings confirm that this method can effectively quantify urinary VOC metabolites in a population exposed to volatile organics. Published by Elsevier B.V.