A triple color FISH technique for mouse chromosome identification
A triple color FISH technique for mouse chromosome identification
复制标题
DOI：
10.1007/s003350020038
复制
发表时间：
2001-06-01
期刊：
影响因子：
2.5
通讯作者：
Greally, JM
中科院分区：
文献类型：
--
作者：
Henegariu, O;Dunai, J;Greally, JM
本文介绍了一种用于小鼠染色体鉴定的三色荧光原位杂交技术。由于不熟悉小鼠染色体显带技术的实验室对小鼠染色体的鉴定相对困难，小鼠中的FISH定位变得复杂。荧光核型分析可以通过使用复染剂、标记的重复探针或多个参考探针来促进。三色FISH技术允许研究人员在一次实验中将未知的基因组克隆分配到特定的小鼠染色体上，而不需要染色体鉴定。这种策略的基础是，如果两条染色体相对于着丝粒的方向不同，则使用每条染色体上的一对颜色相似的DNA探针来区分两条不同染色体的能力。
This article focuses on a triple color FISH (Fluorescence in situ hybridization) technique for mouse chromosome identification. FISH mapping in mouse is complicated by the relative difficulty of mouse chromosome identification by laboratories not familiar with mouse chromosome banding techniques. Fluorescent karyotyping can be facilitated by the use of counterstains, labeled repetitive probes or multiple reference probes. The triple color FISH technique allows researchers to assign an unknown genomic clone to a specific mouse chromosome in a single experiment, without the need for chromosome identification. The basis of this strategy is the ability to distinguish two different chromosomes using a pair of similarly colored DNA probes on each chromosome, if their orientation relative to the centromere differs.