Reactivation of chlamydial genital tract infection in mice
Reactivation of chlamydial genital tract infection in mice
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DOI：
10.1128/iai.65.6.2067-2073.1997
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发表时间：
1997-06-01
影响因子：
3.1
通讯作者：
Byrne, GI
中科院分区：
文献类型：
--
作者：
Cotter, TW;Miranpuri, GS;Byrne, GI
开发了一个模型来研究 C3H/HeN (C3H) 和 C57BL/6N (C57) 小鼠生殖道感染沙眼衣原体 MoPn 后的衣原体静止状态。免疫抑制后衣原体脱落的重新激活表明，在原发感染明显清除后，可存活的 MoPn 在生殖道中保留长达 4 或 5 周。环磷酰胺或醋酸可的松治疗都可能导致重新激活，但环磷酰胺更有效。然而，在这两种小鼠品系中，任何一种药物重新激活的频率都随着时间的推移而减少。 C57小鼠感染前的黄体酮治疗大大降低了环磷酰胺重新激活的频率，并且与绝大多数这些动物中明显的积液和子宫角扩张的发展相关。这种病理在感染后 5 至 7 周内明显，并且在感染后 110 天期间始终可见。在任何测试条件下，在未经黄体酮治疗的 C57 小鼠或 C3H 小鼠中都没有观察到这些现象。MoPn 的感染剂量没有明显影响该模型定义的任一近交系的再激活频率。
A model was developed to study chlamydial quiescence in C3H/HeN (C3H) and C57BL/6N (C57) mice following genital tract infection by Chlamydia trachomatis MoPn. Reactivation of chlamydial shedding following immunosuppression indicated that viable MoPn remained in the genital tract for up to 4 or 5 weeks after the apparent clearance of a primary infection. Either cyclophosphamide or cortisone acetate treatment could cause reactivation, but cyclophosphamide was more effective. However, the frequency of reactivation by either drug diminished with time in both mouse strains. Progesterone treatment prior to infection of C57 mice greatly reduced the frequency of reactivation by cyclophosphamide and also correlated with the development of marked fluid accumulation and distension of the uterine horns in the vast majority of those animals. This pathology was apparent by 5 to 7 weeks postinfection and was consistently seen through 110 days postinfection. Neither of these phenomena was observed in C57 mice that had not been treated,vith progesterone or in C3H mice under any conditions tested, The infecting dose of MoPn did not clearly influence the frequency of reactivation in either inbred strain as defined by this model.