First report of Sarcocystis pilosa sporocysts in feces from red fox, Vulpes vulpes schrencki, in Hokkaido, Japan
First report of Sarcocystis pilosa sporocysts in feces from red fox, Vulpes vulpes schrencki, in Hokkaido, Japan
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DOI：
10.1016/j.ijppaw.2019.12.001
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发表时间：
2020-04-01
影响因子：
1.8
通讯作者：
Yagi, Kinpei
中科院分区：
文献类型：
--
作者：
Irie, Takao;Uraguchi, Kohji;Yagi, Kinpei
在日本北海道的野生梅花鹿（Cervus nippon yesoensis）中，各种类型的肉囊非常普遍，根据形态学和分子特征鉴定出4种：S. ovalis、S. pilosa、S. tarandi-like和S. truncata-like。卵圆线虫的最终宿主是鸦科动物，但其他物种的宿主尚未确定。为了确定这些物种的最终宿主，我们在北海道东部收集了65份红狐（Vulpes Vulpes schrencki）的粪便样本，并采用改良的蔗糖浮选法检测了它们的粪便孢子囊。其中一份粪便样品中含有典型的肉囊菌孢子囊，根据18S核糖体RNA和细胞色素c氧化酶亚基I基因序列鉴定为毛囊菌。这是首次在野外鉴定到毛孢孢子囊。这些结果表明，赤狐是毛孢菌的最终宿主，赤狐是北海道鹿毛孢菌感染的来源之一。
Sarcocysts of various Sarcocystis spp. are highly prevalent in wild sika deer, Cervus nippon yesoensis, in Hokkaido, Japan, and four species have been identified based on morphological and molecular characteristics: S. ovalis, S. pilosa, S. tarandi-like, and S. truncata-like. The definitive hosts of S. ovalis are corvids, but the hosts of the other species have not yet been identified. Aiming to determine the definitive hosts of these species, we collected 65 red fox (Vulpes vulpes schrencki) fecal samples in eastern Hokkaido and examined them for fecal sporocysts using a modified sucrose flotation method. One fecal sample contained typical Sarcocystis sporocysts, which were identified as S. pilosa based on 18S ribosomal RNA and cytochrome c oxidase subunit I gene sequences. This is the first identification of S. pilosa sporocysts in the wild. These findings indicate that red foxes serve as a definitive host of S. pilosa, and that red foxes constitute a source of S. pilosa infection for deer in Hokkaido.