VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector Impurities.
VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector Impurities.
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DOI：
10.3389/fimmu.2021.657795
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发表时间：
2021
影响因子：
7.3
通讯作者：
Paulk NK
中科院分区：
文献类型：
--
作者：
Rumachik NG;Malaker SA;Paulk NK
Progress in recombinant AAV gene therapy product and process development has advanced our understanding of the basic biology of this critical delivery vector. The discovery of rAAV capsid post-translational modifications (PTMs) has spurred interest in the field for detailed rAAV-specific methods for vector lot characterization by mass spectrometry given the unique challenges presented by this viral macromolecular complex. Recent concerns regarding immunogenic responses to systemically administered rAAV at high doses has highlighted the need for investigators to catalog and track potentially immunogenic vector lot components including capsid PTMs and PTMs on host cell protein impurities. Here we present a simple step-by-step guide for academic rAAV laboratories and Chemistry, Manufacturing and Control (CMC) groups in industry to perform an in-house or outsourced bottom-up mass spectrometry workflow to characterize capsid PTMs and process impurities.
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影响因子：
10.1
作者：
Malaker SA;Penny SA;Steadman LG;Myers PT;Loke JC;Raghavan M;Bai DL;Shabanowitz J;Hunt DF;Cobbold M
通讯作者：
Cobbold M
影响因子：
4.2
作者：
Alexander, IE;Russell, DW;Miller, AD
通讯作者：
Miller, AD
DOI：
10.1016/j.omtm.2020.05.018
发表时间：
2020-09-11
影响因子：
4.7
作者：
Rumachik, Neil G.;Malaker, Stacy A.;Paulk, Nicole K.
通讯作者：
Paulk, Nicole K.
影响因子：
4.4
作者：
Riley, Nicholas M.;Malaker, Stacy A.;Bertozzi, Carolyn R.
通讯作者：
Bertozzi, Carolyn R.
DOI：
10.1016/j.jpba.2020.113481
发表时间：
2020-09-10
影响因子：
3.4
作者：
Liu, Anita P.;Patel, Shailin K.;Li, Ning
通讯作者：
Li, Ning