Dynamic assessment of hexose monophosphate shunt activity in the intact rabbit lens by proton NMR spectroscopy.
Dynamic assessment of hexose monophosphate shunt activity in the intact rabbit lens by proton NMR spectroscopy.
复制标题
通过质子核磁共振波谱动态评估完整兔晶状体中己糖单磷酸分流活性。
DOI：
10.1016/s0006-291x(86)80390-4
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Schleich,T
中科院分区：
文献类型：
--
作者：
Willis,JA;Williams,WF;Schleich,T
质子核磁共振技术被证明是为了确定在完整的孵育的兔晶状体中，己糖单磷酸分流对葡萄糖代谢的实时贡献。这种测量需要组织在添加了[1-13C]葡萄糖的介质中孵育，并依赖于乳酸甲基位置上13C标记的存在，这通过13C-1H自旋-标量耦合的方式产生卫星共振。提出了进行测量所需的假设条件。对于在控制条件下保持的镜片，确定了对应于5%己糖单磷酸分流活性的基础水平。在已知的刺激分流的条件下，观察到活动增加了8倍。
A proton nuclear magnetic resonance technique is demonstrated for ascertaining the real-time contribution of the hexose monophosphate shunt to glucose metabolism in the intact incubated rabbit lens. This measurement requires incubation of the tissue in medium supplemented with [1-13C]glucose, and depends on the presence of the13C label in the methyl position of lactate which creates satellite resonances by way of13C-1H spin-spin scalar coupling. The assumptions required to make the measurement are presented. For lenses maintained under control conditions, a basal level corresponding to 5% hexose monophosphate shunt activity was determined. An eight-fold increase in activity was observed under conditions known to stimulate the shunt.