The Immunomodulatory Activity of Jacaric Acid, a Conjugated Linolenic Acid Isomer, on Murine Peritoneal Macrophages.
The Immunomodulatory Activity of Jacaric Acid, a Conjugated Linolenic Acid Isomer, on Murine Peritoneal Macrophages.
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DOI：
10.1371/journal.pone.0143684
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Leung KN
中科院分区：
文献类型：
--
作者：
Liu WN;Leung KN
本研究旨在证明共轭亚麻酸(CLNA)异构体--花青酸对小鼠腹腔巨噬细胞的免疫调节作用。中性红摄取实验结果显示，菠萝酸对硫代乙醇酸盐诱导的小鼠腹膜巨噬细胞无明显的细胞毒性作用，而CyQuant®核因子细胞增殖分析试剂盒检测其对T细胞淋巴瘤MBL-2细胞的细胞抑制活性显著增强。流式细胞仪分析表明，印迹草酸能增强巨噬细胞的内吞活性，增加细胞内超氧阴离子的产生。此外，茉莉酸处理的巨噬细胞表现出一氧化氮的产生增加，并伴随着诱导型一氧化氮合酶蛋白表达水平的增加。此外，几种促炎细胞因子，包括干扰素-γ、白介素1β和肿瘤坏死因子-α的分泌上调。综上所述，我们的结果表明，天然存在的CLNA异构体--菠萝酸在体外对小鼠腹膜巨噬细胞具有免疫调节活性，提示这种CLNA异构体可能作为一种免疫增强剂，用于治疗一些毒性最小、副作用较少的免疫疾病。
This study aims at demonstrating the immunomodulatory property of jacaric acid, a conjugated linolenic acid (CLNA) isomer that is present in jacaranda seed oil, on murine peritoneal macrophages. Our results showed that jacaric acid exhibited no significant cytotoxicity on the thioglycollate-elicited murine peritoneal macrophages as revealed by the neutral red uptake assay, but markedly increased their cytostatic activity on the T-cell lymphoma MBL-2 cells as measured by the fluorometric CyQuant® NF Cell Proliferation Assay Kit. Flow cytometric analysis indicated that jacaric acid could enhance the endocytic activity of macrophages and elevated their intracellular production of superoxide anion. Moreover, jacaric acid-treated macrophages showed an increase in the production of nitric oxide which was accompanied by an increase in the expression level of inducible nitric oxide synthase protein. In addition, the secretion of several pro-inflammatory cytokines, including interferon-γ, interleukin-1β and tumor necrosis factor-α, was up-regulated. Collectively, our results indicated that the naturally-occurring CLNA isomer, jacaric acid, could exhibit immunomodulating activity on the murine peritoneal macrophages in vitro, suggesting that this CLNA isomer may act as an immunopotentiator which can be exploited for the treatment of some immunological disorders with minimal toxicity and fewer side effects.