Progesterone inhibition of mouse uterine epithelial proliferation.
Progesterone inhibition of mouse uterine epithelial proliferation.
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黄体酮抑制小鼠子宫上皮增殖。
DOI：
10.1677/joe.0.0590205
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
L. Martin
中科院分区：
文献类型：
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作者：
R. M. Das;L. Martin
雌二醇刺激卵巢切除小鼠的子宫腔上皮细胞快速连续地经历两轮细胞分裂（Das，1972；Martin、Finn & Trinder，1973）。与雌激素一起注射的黄体酮会阻止一些细胞进入第一轮增殖，而不影响其余细胞的 G1、S 或 G2，但会阻止这些细胞进入第二轮。这表明细胞仅在 G1 早期对孕酮敏感 (Martin, Das & Finn, 1973)。在本实验中，我们试图更详细地描述敏感期。如前所述（Das，1972），随机饲养的白化小鼠被切除卵巢并进行免疫接种。启动后五天，它们被分成十组，每组三人。所有受试者均在0小时皮下注射0\m=.\1ml花生油中的50ng雌二醇，并在15小时腹膜内注射25μg=m\Ci[3H]胸苷（[3H]Thd；sp.act.5 Ci/mm，Amersham）0\m=.\1ml 0\m=.\9%NaCl溶液。第1组还在0小时时皮下注射花生油中的1mg黄体酮，第2-9组在图1所示的时间接受单次注射1mg黄体酮，第10组仅在0小时时接受赋形剂注射。所有小鼠
Oestradiol stimulates uterine luminal epithelial cells of ovariectomized mice to pass through two rounds of cell division in quick succession (Das, 1972; Martin, Finn & Trinder, 1973). Progesterone injected with the oestrogen blocks entry of some cells into the first round of proliferation without affecting G1, S or G2 in the remainder, but blocks entry of these cells into the second round. This suggested that cells were only sensitive to progesterone in early G1 (Martin, Das & Finn, 1973). In the present experiment we attempted to delineate the sensitive period in greater detail. Randomly bred albino mice were ovariectomized and primed as described previously (Das, 1972). Five days after priming they were separated into ten groups of three. All were given 50 ng oestradiol subcutaneously in 0\m=.\1ml arachis oil at 0 h and 25 \g=m\Ci[3H]thymidine ([3H]Thd; sp. act. 5 Ci/mm, Amersham) intraperitoneally in 0\m=.\1ml 0\m=.\9%NaCl solution at 15 h. Group 1 also received 1 mg progesterone sub¬ cutaneously in arachis oil at 0 h, groups 2-9 received single injections of 1 mg pro¬ gesterone at the times indicated in Fig. 1, and group 10, vehicle only at 0 h. All mice