Hydrogen sulfide derived from periadventitial adipose tissue is a vasodilator
Hydrogen sulfide derived from periadventitial adipose tissue is a vasodilator
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DOI：
10.1097/hjh.0b013e328330a900
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发表时间：
2009-11-01
影响因子：
4.9
通讯作者：
Geng, Bin
中科院分区：
文献类型：
--
作者：
Fang, Liping;Zhao, Jing;Geng, Bin
研究背景近年来发现，外膜周围脂肪组织（PAT）分泌的生物活性因子在血管张力调节中起重要作用。硫化氢（H2S），一种新的心血管气体递质，以钙依赖性和非内皮依赖性的方式通过K-ATP通道舒张血管。方法我们首先鉴定了大鼠PAT脂肪细胞中表达的内源性H2S产生的关键酶胱硫醚酶（CSE）和PAT释放的H2S。结果CSE抑制剂DL-炔丙基甘氨酸和/或β-氰基-L-丙氨酸在很大程度上阻断了对主动脉环的血管舒张作用（去除外膜），由PAT诱导，PAT的培养基或从PAT分离的脂肪细胞。苯丙氨酸、5-羟色胺和血管紧张素II抑制了主动脉介质中内源性H2S的产生，但增加了PAT的释放。随着年龄的增长，主动脉和PAT内源性H2S生成减少，CSE蛋白水平升高。在腹主动脉缩窄诱导的高血压大鼠中，PAT中H2S生成和CSE蛋白表达显著增加，而主动脉组织中无。结论PAT可内源性产生H2S，H2S可能是一种脂肪细胞源性舒张因子，参与了高血压的发病过程。J Hypertens 27：2174-2185（C）2009 Wolters Kluwer Health|利平科特威廉姆斯&威尔金斯。
Background Recently, periadventitial adipose tissue (PAT) was found to secrete bioactive factors playing an important role in the regulation of vascular tension. Hydrogen sulfide (H2S), a novel cardiovascular gasotransmitter, relaxes vessels through the K-ATP channel in a calcium-dependent and endothelial-independent manner.Methods We first identified the endogenous H2S-generated key enzyme cystathionase (CSE) expressed in adipocytes of PAT and H2S released from PAT in rats.Results The CSE inhibitors DL-propargylglycine and/or beta-cyano-L-alanine largely blocked the vasorelaxing effects on aorta rings (removed adventitia), induced by PAT, the culture medium of PAT or isolated adipocytes from PAT. Phenylephrine, serotonin and angiotensin II inhibited endogenous H2S production from the aortic medium but increased its release from PAT. Endogenous H2S generated in the aorta and PAT was decreased but the level of CSE protein was increased with the aging of rats. In rats with hypertension induced by abdominal aortic banding, H2S generation and CSE protein expression were significantly increased in PAT but not aortic tissues. Transplanting PAT into periadventitia of stenotic aortas ameliorated the elevated arterial blood pressure and decreased angiotensin II level in aorta.Conclusion These results suggested PAT could endogenously generate H2S, which might act as an adipocyte-derived relaxing factor and contribute to the pathogenesis of hypertension. J Hypertens 27: 2174-2185 (C) 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins.