Genome-wide profiling of segmental-regulated transcriptomes in human epididymis using oligo microarray
Genome-wide profiling of segmental-regulated transcriptomes in human epididymis using oligo microarray
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DOI：
10.1016/j.mce.2005.12.041
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发表时间：
2006-05-16
影响因子：
4.1
通讯作者：
Zhang, Yong-Lian
中科院分区：
文献类型：
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作者：
Zhang, Jin-Song;Liu, Qiang;Zhang, Yong-Lian
精子在通过附睾过程中的成熟取决于区域化的基因表达，它维持了附睾小管内逐渐变化的环境。为了确定驱动精子顺序成熟的基因，我们使用代表大约整个人类基因组的Affymetrix人类基因组U133 + 2.0微阵列分析了一个可生育的年轻男性供体附睾中区域调控的基因表达模式。在整个附睾中鉴定了15000多个转录本，几乎占阵列总数的三分之一。其中，在附睾三个区域均检出的占65%，仅在一个区域检出的占2.6%，在两个区域检出的占32.4%。在附睾头（264）、体（61）和尾（81）中观察到的区域特异性转录本被进一步归类为经验确定的报告基因或ESTs。本研究首次揭示了一些区域特异性基因在人附睾中的表达。原始数据将在上海科技数据库（http://www.scbit.org/human_epididymis_transcriptomes）上公开。2005爱思唯尔爱尔兰有限公司版权所有。
Sperm maturation during passage through the epididymis depends on regionalized gene expression which maintains the progressively changing environment within the epididymal tubule. Towards defining the genes that drive the sequential maturation of spermatozoa, we profiled regionally regulated gene expression pattern in the epididymis of a fertile young male donor using Affymetrix human genome U133 plus 2.0 microarray representing approximately the whole human genome. Over 15 000 transcripts, almost one-third of the total on the array were identified in whole epididymis. Among them, 65% were detected in all three regions of the epididymis, 410 or 2.6% were present only in one region and the remaining 32.4% were distributed in two regions. Region-specific transcripts observed in caput (264), corpus (61) and cauda (81) epididymides were further classified as empirically determined reported genes or ESTs. This study revealed for the first time, the expression in human epididymis of a number of region-specific genes. The original data will be made publicly available on the Shanghai Science and Technology Database (http://www.scbit.org/human_epididymis_transcriptomes). (c) 2005 Elsevier Ireland Ltd. All rights reserved.