IN-VITRO ANALYSIS OF AH RECEPTOR DOMAINS INVOLVED IN LIGAND-ACTIVATED DNA RECOGNITION
IN-VITRO ANALYSIS OF AH RECEPTOR DOMAINS INVOLVED IN LIGAND-ACTIVATED DNA RECOGNITION
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DOI：
10.1073/pnas.90.18.8566
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发表时间：
1993-09-15
影响因子：
11.1
通讯作者：
BRADFIELD, CA
中科院分区：
文献类型：
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作者：
DOLWICK, KM;SWANSON, HI;BRADFIELD, CA
Ah受体（AHR）是介导2，3，7，8-四氯二苯并-p-二恶英作用的碱性螺旋-环-螺旋蛋白。在这份报告中，我们描述了一个兔网织红细胞系统，允许功能表达的AHR和它的二聚体的合作伙伴，AHR核转位蛋白（ARNT）。通过使用这种体外系统，我们能够重建激动剂结合AHR和激动剂诱导的AHR-ARNT识别同源DNA增强子序列。AHR缺失突变体的表达揭示了负责配体和DNA识别的N-末端结构域和在激动剂诱导的DNA识别中发挥作用的C-末端结构域的位置。
The Ah receptor (AHR) is a basic helix-loop-helix protein that mediates the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin. In this report, we describe a rabbit reticulocyte system that allows functional expression of both the AHR and its dimeric partner, the AHR nuclear translocator protein (ARNT). By using this in vitro system, we were able to reconstitute agonist binding to the AHR and agonist-induced AHR-ARNT recognition of a cognate DNA enhancer sequence. Expression of AHR deletion mutants revealed the location of N-terminal domains responsible for ligand and DNA recognition and C-terminal domains that play roles in agonist-induced DNA recognition.