Identification of Blue Pigment Formed in a D-Xylose-Glycine Reaction System.
Identification of Blue Pigment Formed in a D-Xylose-Glycine Reaction System.
复制标题
D-木糖-甘氨酸反应体系中形成的蓝色色素的鉴定。
DOI：
10.1271/bbb.63.1512
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
H. Kato
中科院分区：
文献类型：
--
作者：
F. Hayase;Y. Takahashi;S. Tominaga;M. Miura;T. Gomyo;H. Kato
将d -木糖（1 M）、甘氨酸（0.1 M）和碳酸氢钠（0.1 M）溶于pH为8.1的60%乙醇水溶液中，在26.5℃、氮气置换的暗室中放置2天。通过阴离子交换和凝胶过滤色谱，从蓝色溶液中分离纯化了蓝色色素。蓝色颜料命名为Blue- m1（蓝色美拉德反应中间体-1），鉴定为5-{[1,4-（二羧基甲基）-5-（2,3-二羟丙基）-2-吡咯罗[3,2-b]吡咯基]甲基}-1,4-（二羧基甲基）-2-（1,2,3-三羟丙基）-吡咯罗[3,2-b]吡咯-铕。Blue-M1应该是黄色吡咯-2-甲醛化合物的二聚体。易于与黄色化合物反应的Blue-M1具有聚合活性，表明它是形成类黑素的重要美拉德反应中间体。
D-Xylose (1 M), glycine (0.1 M), and sodium hydrogencarbonate (0.1 M) were dissolved in aqueous 60% ethanol at pH 8.1 and left at 26.5°C for 2 days in a dark room under nitrogen displacement. Blue pigment was isolated and purified from the blue solution by anionic exchange and gel filtration chromatographies. Blue pigment which was designated Blue-M1 (blue Maillard reaction intermediate-1) was identified as 5-{[1,4-(dicarboxymethyl)-5-(2,3-dihydroxypropyl)-2-pyrrolo[3,2-b]pyrrolyl]methine}-1,4-(dicarboxymethyl)-2-(1,2,3-trihydroxypropyl)-pyrrolo[3,2-b]pyrroly-lium. Blue-M1 is supposed to be a dimer of yellow colored pyrrolopyrrole-2-carboxaldehyde compounds. Blue-M1 that reacts readily to yellow compounds has a polymerizing activity, suggesting it is an important Maillard reaction intermediate through the formation of melanoidins.