Nucleoside diphosphate kinase III is localized to the inter-membrane space in plant mitochondria
Nucleoside diphosphate kinase III is localized to the inter-membrane space in plant mitochondria
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DOI：
10.1016/s0014-5793(01)03069-1
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发表时间：
2001-11-16
期刊：
影响因子：
3.5
通讯作者：
Millar, AH
中科院分区：
文献类型：
--
作者：
Sweetlove, LJ;Mowday, B;Millar, AH
在植物中发现了三种类型的核苷二磷酸激酶（NDPKs），但这些蛋白质的细胞内区室化并不确定，特别是最近发现的III型蛋白质的位置。通过从马铃薯和拟南芥中分离植物线粒体，通过2D凝胶电泳显示蛋白质谱，并通过质谱鉴定，我们提出了第一个直接的证据表明，III型蛋白定位于植物线粒体的膜间隙。NDPK III的可能的代谢功能进行了讨论，根据其亚细胞定位。(C)2001年由Elsevier Science B.V.代表欧洲生物化学学会联合会出版。
Three types of nucleoside diphosphate kinases (NDPKs) are found in plants but the intra-cellular compartmentation of these proteins is not certain, especially the location of the recently identified type III proteins. Through the fractionation of plant mitochondria from potato and Arabidopsis, display of protein profiles by 2D gel electrophoresis, and identification by mass spectrometry, we present the first direct evidence that type III proteins are localized in the inter-membrane space of plant mitochondria. The possible metabolic functions of NDPK III are discussed in light of its sub-cellular localization. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.