Decreased expression of connexin-30 and aberrant expression of connexin-26 in human head and neck cancer.
Decreased expression of connexin-30 and aberrant expression of connexin-26 in human head and neck cancer.
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发表时间：
2007-07
影响因子：
2
通讯作者：
Hiroyuki Ozawa;T. Matsunaga;Kazusaku Kamiya;Y. Tokumaru;M. Fujii;T. Tomita;K. Ogawa
中科院分区：
文献类型：
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作者：
Hiroyuki Ozawa;T. Matsunaga;Kazusaku Kamiya;Y. Tokumaru;M. Fujii;T. Tomita;K. Ogawa
研究背景连接蛋白与肿瘤发生的关系已在多种器官和细胞系中进行了研究，连接蛋白目前被认为是肿瘤抑制因子。材料与方法应用免疫组织化学方法检测连接蛋白26（Cx 26）和连接蛋白30（Cx 30）在头颈部癌组织及癌旁正常组织中的表达。此外，这些连接蛋白的表达与细胞凋亡和细胞分化进行了研究。结果胃癌组织中Cx 30的表达明显低于正常黏膜组织，而Cx 26的表达与正常黏膜组织无明显差异。Cx 26和Cx 30在癌细胞中的细胞内分布模式在癌部位之间不同。在癌组织中，Cx 26和Cx 30的表达与凋亡（由TdT介导的dUTP-生物素缺口末端标记法（TUNEL）反应评估）或细胞分化（由免疫反应评估到外皮蛋白）无关。结论连接蛋白在头颈癌的发生发展中可能起一定作用。
BACKGROUND The relation of connexins and carcinogenesis has been studied in various organs and cell lines, with connexins currently being considered tumor suppressors. MATERIALS AND METHODS The expression of connexin-26 (Cx26) and connexin-30 (Cx30) in human head and neck carcinomas, as well as in adjacent normal mucosa, was evaluated by immunohistochemistry. Furthermore, the expression of these connexins with regard to apoptosis and cell differentiation was investigated. RESULTS In the cancer tissues, Cx30 expression was drastically reduced compared to apparently normal mucosa while Cx26 expression was almost the same. The intracellular distribution patterns of Cx26 and Cx30 in cancer cells differed between cancer locations. In the cancer tissues, expression of Cx26 and Cx30 was not related to apoptosis assessed by TdT-mediated dUTP-biotin nick end labeling (TUNEL) reaction or to cell differentiation assessed by immunoreaction to involucrin. CONCLUSION The present results suggest that connexins may play a role in the carcinogenesis of head and neck cancer.