Characterization of a New Aedes albopictus (Diptera: Culicidae)-Wolbachia pipientis (Rickettsiales: Rickettsiaceae) Symbiotic Association Generated by Artificial Transfer of the wPip Strain From Culex pipiens (Diptera: Culicidae)
Characterization of a New Aedes albopictus (Diptera: Culicidae)-Wolbachia pipientis (Rickettsiales: Rickettsiaceae) Symbiotic Association Generated by Artificial Transfer of the wPip Strain From Culex pipiens (Diptera: Culicidae)
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DOI：
10.1603/me09140
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发表时间：
2010-03-01
影响因子：
2.1
通讯作者：
Dobson, Stephen L.
中科院分区：
文献类型：
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作者：
Calvitti, Maurizio;Moretti, Riccardo;Dobson, Stephen L.
沃尔巴克氏体是一种母系遗传的内共生体，可对昆虫和其他无脊椎动物宿主产生多种影响，促进幼稚宿主群体的入侵。其中一个影响是一种称为细胞质不相容性（CI）的不育形式，当雌性与携带不同沃尔巴克氏体菌株的雄性交配时，雌性会被有效地绝育。细胞质不相容的雄性的重复大量释放可以成为抑制昆虫种群的工具。在这里，我们尝试用来自淡色库蚊（L.）（双翅目：库蚊科）的新沃尔巴克氏体菌株感染白纹伊蚊（Skuse）（双翅目：库蚊科）菌株，人工剥夺了天然沃尔巴克氏体感染。进一步的实验旨在研究新感染对伊蚊的影响。白纹伊蚊适应性并评估影响感染动态的关键参数，包括 CI 水平和母系遗传。利用胚胎显微注射，在伊蚊中成功建立了新的沃尔巴克氏体菌株。白纹伊蚊。杂交表现出自然感染个体和转染个体之间的双向 Cl 模式。具体而言，在具有不同感染状态的转染雄性和雌性之间的所有杂交中，基本上不存在卵孵化（即，Cl非常高）。此外，自然感染的伊蚊。白纹伊蚊雄性与转染的雌性不相容。母系遗传接近100%。此外，新的感染并不影响未成熟和成虫的存活率，但它显着降低了雌性的生育力和卵孵化率。讨论了与新 Ae 的潜在用途相关的结果。白纹伊蚊-沃尔巴克氏体共生关联作为研究和开发基于 CI 的策略来抑制这一重要病虫害媒介种群的合适系统。
Wolbachia is a maternally inherited endosymbiont inducing various effects in insects and other invertebrate hosts that facilitate the invasion of naive host populations. One of the effects is a form of sterility known its cytoplasmic incompatibility (CI) through which females are effectively sterilized when they mate with males harboring a different Wolbachia strain. The repeated mass release of cytoplasmically incompatible males can be a tool to Suppress insect populations. Here, we attempt to infect an Aedes albopictus (Skuse) (Diptera: Culicidae) strain, artificially deprived of the natural Wolbachia infection, with a new Wolbachia strain from Culex pipiens (L.) (Diptera: Culicidae). Further experiments were designed to study the effects of the new infection on Ae. albopictus fitness and evaluate key parameters that affect infection dynamics, including CI level and maternal inheritance. Using embryonic microinjection, the new Wolbachia strain was successfully established in Ae. albopictus. Crosses demonstrated a pattern of bidirectional Cl between naturally infected and transinfected individuals. Specifically, egg hatch was essentially absent (i.e., Cl was very high) in all crosses between the transinfected males and females with it different infection status. Furthermore, naturally infected Ae. albopictus males were incompatible with the transinfected females. Maternal inheritance was close to 100%. Moreover, the new infection did not affect immature and adult survivorship, but it significantly reduced female fecundity and egg hatch rate. The results are discussed in relation to the potential use of the new Ae. albopictus-Wolbachia symbiotic association as a suitable system for the study and development of CI-based strategies for Suppressing populations of this important pest and disease vector.