Detection rates of TT virus DNA in serum of umbilical cord blood, breast milk and saliva
Detection rates of TT virus DNA in serum of umbilical cord blood, breast milk and saliva
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DOI：
10.1620/tjem.191.203
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发表时间：
2000-08-01
影响因子：
2.2
通讯作者：
Wada, Y
中科院分区：
文献类型：
--
作者：
Goto, K;Sugiyama, K;Wada, Y
迄今为止，TT病毒（TTV）的母婴传播途径尚未完全阐明。本研究探讨了TTV DNA的检出率。在日本孕妇的血清和分娩时的脐带血中，以及产后一个月的母亲的唾液和母乳中。引物来源于著名的翻译区N22 （N22系统）和非翻译区（UTR系统）。孕妇血清TTV DNA检出率N22为11.9% (19/160)，UTR为72.4%（55/76）。使用N22系统检测脐带血样本（0/160）未检测到TTV DNA，而使用UTR系统检测的样本中有11.8%（7/76）检测到TTV DNA。使用N22系统，在母乳中未检测到TTV DNA，但在唾液中检测到。然而，使用UTR系统，在两个标本中检测到TTV DNA。这些结果表明，一些婴儿在分娩时通过脐带血或母乳或唾液垂直感染了TTV。然而，需要进一步的研究来证实这一假设。
To date, the routes of mother-to-infant transmission of TT virus (TTV) have not been fully elucidated. The present study examines the detection rates of TTV DNA. in the serum of pregnant Japanese women and in cord blood at the time of delivery, as well as in the saliva and breast milk of mothers one-month postpartum. Primers derived from the well-known translated region N22 (N22 system), as well as the untranslated region (UTR system) were used. The prevalence of TTV DNA in the serum of pregnant women was found to be 11.9% (19/160) using the N22 system and 72.4% (55/76) using the UTR system. No TTV DNA was detected in the cord blood samples (0/160) when the N22 system was used for detection but TTV DNA was detected in 11.8% (7/76) of samples studied with the UTR system. Using the N22 system, TTV DNA was not detected in breast milk, but was detected in saliva. However using the UTR system, TTV DNA was detected in both specimens. These results imply that some babies are vertically infected with TTV via cord blood at the time of delivery or via breast milk or saliva. However, further research is necessary to confirm this hypothesis.