Sequence gel reading with a portable computer.
Sequence gel reading with a portable computer.
复制标题
使用便携式计算机进行测序凝胶读数。
DOI：
10.1093/nar/14.4.1917
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发表时间：
1986
影响因子：
14.9
通讯作者：
Hutchison3rd,CA
中科院分区：
文献类型：
--
作者：
Hutchison3rd,CA
传统上，DNA序列数据被转录到纸上，然后输入计算机进行进一步分析。这是一个乏味的、容易出错的过程，通常是阻碍分析所有可用数据的瓶颈。该问题的一种解决方案是使用数字化仪直接输入数据（Lautenberger，JA（1982）Nucl. Acids Res.10，27-30）。本报告描述了一种快速、方便和经济的替代方案。可使用便携式计算机（Radio Shack TRS-80 Model 100）来读取测序凝胶。该计算机在尺寸和重量上与”分子克隆，实验室手册”（Maniatis，T.，Fritsch，EF，and Sambrook，J.（1982）冷泉港实验室）。可移植性是一个有价值的特性，因为它允许在方便的位置（如个人的办公桌或家中）输入数据。数据很容易被转移到一个更大的计算机上，以便通过诸如”DB系统”（Staden，R.（1982）Nucl. Acids Res.10，4731-4751）。下列程序可用（发送IBM-PC或TRS-80 Model 100软盘）：序列输入P-四个相邻的键按顺序对应于序列阶梯的四个通道。当输入序列时，对应于四个通道的四个音调响起。当输入数据时，读者的眼睛仍然集中在凝胶上。即使是非打字员也能实现准确的数据输入。校对和编辑模式允许回放先前输入的序列，每个碱基在计算机屏幕上显示为一个字符，也可以显示为一个音调。这允许在校对期间在测序凝胶上进行视觉集中。BATCAT-将单个序列凝胶读数连接到文件中，以便使用BATIN程序将批次输入Staden DB系统。部分由NIH资助AI 08998。
DNA sequence data is traditionally transcribed onto paper, then typed into a anputer for further analysis. This is a tedious, error prone process, which is oftena bottleneck that discouragesanalysis of all the available data. One solution to the problem is the use of a digitizer for direct dataentry (Lautenberger, JA (1982) Nucl. Acids Res. 10, 27-30). This report describes a rapid, convenient, and economical alternative. A portable computer (Radio Shack TRS-80 Model 100) may be used to read sequencing gels. This computer is quite similar in size and weight to" Molecular Cloning, A Laboratory Manual"(Maniatis, T., Fritsch, EF, and Sambrook, J.(1982) Cold Spring Harbor Laboratory). Portability is a valuable feature since it allcMws data entry at convenient locations such as an individual's desk, or at home. Data is easily transferred to a larger computer for assembly by programs such as the" DB System"(Staden, R.(1982) Nucl. Acids Res. 10, 4731-4751). The following programs are available (send an IBM-PC or TRS-80 Model 100 diskette): SEQINP-Four adjacent keys are assigned to correspond, in order, to the four lanes of a sequence ladder. As the sequence is entered, four musical tones are sounded corresponding to the four lanes. The reader's eyes remain trained on the gel as data is entered. Even non-typists achieve accurate data entry. A proofreading and editingmode allows play-back of a previously entered sequence, with each base displayed as a character on the computer screen and also as a musical tone. This allows visual concentration on thesequencing gel during proofreading. BATCAT-concatenates individual sequence gel readings into a file for batch entry to the Staden DB system using the BATIN program. Supported in part by NIH grant AI08998.