Tools and methods to analyze autophagy in C. elegans
Tools and methods to analyze autophagy in C. elegans
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DOI：
10.1016/j.ymeth.2014.11.019
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发表时间：
2015-03-15
期刊：
影响因子：
4.8
通讯作者：
Legouis, Renaud
中科院分区：
文献类型：
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作者：
Jenzer, Celine;Simionato, Elena;Legouis, Renaud
长期以来，自噬主要在酵母或哺乳动物细胞系中进行研究，并且已经很好地描述了用于分析这些模型中的自噬的测定。最近，在多细胞生物体中研究了自噬在各种生理功能中的参与。自噬通量的改变涉及发育过程、对应激条件的抗性、衰老、细胞死亡和多种病理学。因此，使用动物模型对于理解不同细胞类型和整个生命过程中的这些过程至关重要。十年来，秀丽隐杆线虫已经成为分析生理或病理背景下自噬的强大模型。在这篇文章中，我们提出了一些已建立的方法和新兴的工具，可用于监测和操纵自噬在C。elegans，并讨论其优点和局限性。(C)2014 Elsevier Inc. All rights reserved.
For a long time, autophagy has been mainly studied in yeast or mammalian cell lines, and assays for analyzing autophagy in these models have been well described. More recently, the involvement of autophagy in various physiological functions has been investigated in multicellular organisms. Modification of autophagy flux is involved in developmental processes, resistance to stress conditions, aging, cell death and multiple pathologies. So, the use of animal models is essential to understand these processes in the context of different cell types and during the whole life. For ten years, the nematode Caenorhabditis elegans has emerged as a powerful model to analyze autophagy in physiological or pathological contexts. In this article, we present some of the established approaches and the emerging tools available to monitor and manipulate autophagy in C. elegans, and discuss their advantages and limitations. (C) 2014 Elsevier Inc. All rights reserved.