Pressure-driven perfusion culture microchamber array for a parallel drug cytotoxicity assay
Pressure-driven perfusion culture microchamber array for a parallel drug cytotoxicity assay
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DOI：
10.1002/bit.21836
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发表时间：
2008-08-15
影响因子：
3.8
通讯作者：
Kanamori, Toshiyuki
中科院分区：
文献类型：
--
作者：
Sugiura, Shinji;Edahiro, Jun-ichi;Kanamori, Toshiyuki
本文报道了一种为平行药物细胞毒性测定而开发的压力驱动灌注培养芯片。该装置由具有独立灌注微通道的 8 x 5 细胞培养微室阵列组成。它配备了一个简单的接口，可以方便地使用微量移液器并连接到外部压力源，无需特殊培训即可轻松操作。独特的微室结构经过精心设计，考虑了流体动力学参数，并通过多层光刻和复制成型由聚二甲基硅氧烷制成。微室结构能够实现均匀的细胞装载和灌注培养，而相邻微室之间不会交叉污染。在8 x 5微室阵列中成功进行平行细胞毒性测定，分析七种抗癌药物的细胞毒性作用。压力驱动灌注培养芯片以其简单的接口和精心设计的微流控网络，将有可能成为未来微芯片高通量药物筛选的有利平台。
This article reports a pressure-driven perfusion culture chip developed for parallel drug cytotoxicity assay. The device is composed of an 8 x 5 array of cell culture microchambers with independent perfusion microchannels. It is equipped with a simple interface for convenient access by a micropipette and connection to an external pressure source, which enables easy operation without special training. The unique microchamber structure was carefully designed with consideration of hydrodynamic parameters and was fabricated out of a polydimethylsiloxane by using multilayer photolithography and replica molding. The microchamber structure enables uniform cell loading and perfusion culture without cross-contamination between neighboring microchambers. A parallel cytotoxicity assay was successfully carried out in the 8 x 5 microchamber array to analyze the cytotoxic effects of seven anticancer drugs. The pressure-driven perfusion culture chip, with its simple interface and well-designed microfluidic network, will likely become an advantageous platform for future high-throughput drug screening by microchip.