CONTINUOUS PRODUCTION OF ACETONE AND BUTANOL BY CLOSTRIDIUM-ACETOBUTYLICUM IN A 2-STAGE PHOSPHATE LIMITED CHEMOSTAT
CONTINUOUS PRODUCTION OF ACETONE AND BUTANOL BY CLOSTRIDIUM-ACETOBUTYLICUM IN A 2-STAGE PHOSPHATE LIMITED CHEMOSTAT
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DOI：
10.1007/bf00499955
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
GOTTSCHALK, G
中科院分区：
文献类型：
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作者：
BAHL, H;ANDERSCH, W;GOTTSCHALK, G
在磷酸限（0.74 mM）、pH为4.3的连续培养条件下，葡萄糖发酵生成丁醇、丙酮和乙醇为主要产物。稀释率为D=0.025 h−1，葡萄糖浓度为300 mM时，丁醇和丙酮的最大浓度分别为130 mM和74 mM。20%的葡萄糖留在培养基中。在这些结果的基础上，开发了两阶段连续工艺，其中87.5%的葡萄糖转化为丁醇、丙酮和乙醇。剩下的12.5%是细胞和少量的乙酸和丁酸盐。第一期在D=0.125 h - 1和37°C条件下进行，第二期在D=0.04 h - 1和33°C条件下进行，在磷酸盐限制下分批培养也获得了高产丁醇和丙酮。
WhenClostridium acetobutylicumwas grown in continuous culture under phosphate limitation (0.74 mM) at a pH of 4.3, glucose was fermented to butanol, acetone and ethanol as the major products. At a dilution rate of D=0.025 h−1and a glucose concentration of 300 mM, the maximal butanol and acetone concentrations were 130 mM and 74 mM, respectively. 20% of the glucose remained in the medium. On the basis of these results a two-stage continuous process was developed in which 87.5% of the glucose was converted into butanol, acetone and ethanol. The cells and minor amounts of acetate and butyrate accounted for the remaining 12.5% of the substrate. The first stage was run at D=0.125 h−1and 37° C and the second stage at D=0.04 h−1and 33° C. High yields of butanol and acetone were also obtained in batch culture under phosphate limitation.