The expression of genes modulating programmed cell death in normal human polymorphonuclear neutrophils
The expression of genes modulating programmed cell death in normal human polymorphonuclear neutrophils
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DOI：
10.1006/bbrc.1997.6529
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发表时间：
1997-04-28
影响因子：
3.1
通讯作者：
Yu, CL
中科院分区：
文献类型：
--
作者：
Hsieh, SC;Huang, MH;Yu, CL
正常人多形核中性粒细胞（PMN）寿命短，在细胞凋亡过程中死亡。为了了解PMN细胞凋亡的分子基础，我们采用流式细胞术、Western blot和逆转录辅助聚合酶链反应（RT-PCR）检测凋亡相关基因（Fas、Fas配体、p53、c-myc）和存活相关基因（bcl-2）的表达。我们发现Fas和Fas配体（Fast）在大多数细胞表面表达。但孵育24h后，Fast的消失速度明显快于Fas。P53和bcl-2在大多数细胞的细胞质中也有表达。相比之下，c-myc在PMN中的表达可以忽略不计。在PMN混悬液中加入单克隆抗人Fas抗体（25 μ g/ml）可增强PMN的凋亡，而抗fasl抗体（25 μ g/ml）可抑制PMN的凋亡。提示PMN间Fas- fasl相互作用诱导Fas通路激活是PMN自发凋亡的机制之一。原癌蛋白c-myc在PMN中缺乏表达是其非增殖特性的原因，并可能加剧细胞的自发凋亡。(C) 1997学术出版社。
Normal human polymorphonuclear neutrophils (PMN) have a short life and die in progression via apoptosis. In order to understand the molecular basis of PMN apoptosis, the expression of apoptosis-related (Fas, Fas-ligand, p53, and c-myc) and survival-related (bcl-2) genes was detected by flow cytometry, Western blot and reverse transcription-assisted polymerase chain reaction (RT-PCR). We found that Fas and Fas-ligand (Fast) were expressed on the sur-face of most of the cells. However, the disappearance of Fast was much faster than Fas after 24h incubation. p53 and bcl-2 were also expressed in the cytoplasm of most of the cells. In contrast, the expression of c-myc was negligible in PMN. The addition of monoclonal anti-human Fas antibody (25 mu g/ml) to PMN suspension enhanced whereas anti-FasL antibody (25 mu g/ml) suppressed PMN apoptosis in 48h incubation. These results suggest that the activation of Fas pathway induced by Fas-FasL interaction among PMNs is one of the mechanisms for spontaneous PMN apoptosis. Lack of proto-oncoprotein c-myc expression in PMN is responsible for their non-proliferative property and may aggravate the spontaneous apoptosis of the cells. (C) 1997 Academic Press.