CONTRIBUTION OF CR3, CD11B/CD18 TO CYTOLYSIS BY HUMAN NK CELLS
CONTRIBUTION OF CR3, CD11B/CD18 TO CYTOLYSIS BY HUMAN NK CELLS
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DOI：
10.1016/0161-5890(90)90041-w
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发表时间：
1990-12-01
影响因子：
3.6
通讯作者：
YEFENOF, E
中科院分区：
文献类型：
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作者：
KLEIN, E;DIRENZO, L;YEFENOF, E
补体受体CR3分子在其β链CD18介导的细胞间直接接触中发挥作用。与Fc受体(CD16)类似，CR3也是人类自然杀伤细胞的标志。我们已经证明，用IC3b对NK靶标进行调理可以提高它们的裂解敏感性。可以通过在人血清中孵育某些B和T细胞系来实现调理。CR2的表达是C3片段固定的先决条件。CR2阴性细胞株P3HR1在人血清中诱导表达EBV包膜糖蛋白gp350后，可被调理。C3b或iC3b也可以通过与表面反应性抗体的化学偶联而人工沉积在细胞表面。与巨噬细胞和单核细胞的功能类似，仅通过CR3的iC3b结合位点与调理靶细胞接触似乎不会触发NK功能。我们试图阐明其他CR3配体的功能作用。CD18分子的β链对NK效应是必不可少的。NK靶点似乎不与CR3、CD11bα链上的β-葡聚糖结合表位相互作用。另一方面，通过该表位与适当的配基结合，可以增强其细胞溶解功能。
The complement receptor CR3 molecule functions in direct intercellular contacts mediated by its beta chain, CD18. Similarly to the Fc receptor (CD16), CR3 is a marker of human natural killer cells. We have shown that opsonization of NK targets with iC3b leads to their increased lytic sensitivity. Opsonization could be achieved by incubating certain B and T cells lines in human serum. The expression of CR2 was a prerequisite for C3 fragment fixation. The CR2 negative cell line, P3HR1 could be opsonized by incubation in human serum when induced to express the EBV envelope glycoprotein gp350. C3b or iC3b could also be deposited artificially on cell surfaces by chemical coupling to surface reactive antibodies. Similarly to the function of macrophages and monocytes, contact with opsonized targets exclusively through the iC3b binding site of CR3 did not seem to trigger NK function. We attempted to clarify the functional role of other CR3 ligands. The beta chain of the molecule, CD18, was essential to the NK effect. The NK targets did not seem to interact with the beta-glucan binding epitope on the alpha chain of CR3, CD11b. On the other hand, the cytolytic function could be enhanced through this epitope with the appropriate ligand.