Novel cryo-imaging of the glioma tumor microenvironment reveals migration and dispersal pathways in vivid three-dimensional detail.
Novel cryo-imaging of the glioma tumor microenvironment reveals migration and dispersal pathways in vivid three-dimensional detail.
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DOI：
10.1158/0008-5472.can-11-1553
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发表时间：
2011-09-01
期刊：
影响因子：
11.2
通讯作者：
Brady-Kalnay SM
中科院分区：
文献类型：
--
作者：
Burden-Gulley SM;Qutaish MQ;Sullivant KE;Lu H;Wang J;Craig SE;Basilion JP;Wilson DL;Brady-Kalnay SM
肿瘤微环境中细胞迁移成像的传统方法包括异种移植物的连续切片和标准组织学染色。目前的分子成像技术存在分辨率低和难以通过颅骨成像的问题。在这里，我们展示了如何使用计算机算法来重建从人类神经胶质瘤小鼠异种移植模型获得的组织切片的图像，并可以渲染成3-D图像，提供肿瘤细胞分散的精美解剖细节。我们的研究结果确定了人类LN-229和啮齿动物CNS-1胶质瘤细胞作为有效的系统，以研究胶质瘤肿瘤细胞沿着血管和白色束在体内的高度分散性。这种新的冷冻成像技术提供了一个有价值的工具，以评估针对限制肿瘤细胞的侵袭和扩散的治疗干预。
Traditional methods of imaging cell migration in the tumor microenvironment include serial sections of xenografts and standard histological stains. Current molecular imaging techniques suffer from low resolution and difficulty in imaging through the skull. Here we show how computer algorithms can be used to reconstruct images from tissue sections obtained from mouse xenograft models of human glioma and can be rendered into 3-D images offering exquisite anatomic detail of tumor cell dispersal. Our findings identify human LN-229 and rodent CNS-1 glioma cells as valid systems to study the highly dispersive nature of glioma tumor cells along blood vessels and white matter tracts in vivo. This novel cryo-imaging technique provides a valuable tool to evaluate therapeutic interventions targeted at limiting tumor cell invasion and dispersal.