Transrectal ultrasonic diagnosis of ovarian follicular cysts in goats and treatment with GnRH
Transrectal ultrasonic diagnosis of ovarian follicular cysts in goats and treatment with GnRH
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DOI：
10.1016/j.domaniend.2004.03.006
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发表时间：
2004-08-01
影响因子：
2.1
通讯作者：
Taya, K
中科院分区：
文献类型：
--
作者：
Medan, MS;Watanabe, G;Taya, K
囊性卵巢疾病是导致生育失败的重要原因。本研究的目的是评价经直肠超声作为诊断工具和促性腺激素释放激素（GnRH）作为治疗方法的山羊卵巢卵泡囊肿。如果在间隔5天进行的3次超声检查中，在没有黄体（CL）的情况下检测到直径>10 mm的无回声结构，则认为山羊患有卵泡囊肿。诊断后（第0天），对患有卵巢卵泡囊肿的山羊（n = 5）单次推注10.5 μ g合成GnRH，10天后给予125 μ g前列腺素F2 α（PGF 2 α）。每隔5天采集5份血样，测定孕酮和雌二醇-17 β。为了检测LH峰，每2小时采集一次血样。经直肠超声检查确定排卵率和妊娠。结果表明，经直肠超声检查对诊断卵巢卵泡囊肿是可靠的，卵泡囊肿的平均直径为12.6 +/- 0.4 mm。在诊断卵泡囊肿时（第0天），孕酮和雌二醇-17 β的血浆浓度分别为0.7 +/- 0.2 ng/ml和12.7 +/- 0.9 pg/ml。在给予GnRH后10天，孕酮的浓度增加至4.0 +/- 0.5 ng/ml，表明卵巢卵泡囊肿的黄素化伴随雌二醇-17 β浓度的降低（3.5 +/- 0.4 pg/ml）。给囊山羊注射GnRH导致LH在治疗后2小时内激增。从注射PGF 2 α到排卵前LH峰的时间间隔为62.8 +/- 1.4 h。所有山羊在注射PGF 2 α后55.2 +/- 2.3 h发情，5只山羊中有4只排卵。排卵率为1.5 +/- 0.3。总之，本研究的结果表明，经直肠超声检查是一种可靠的工具，诊断卵巢卵泡囊肿。此外，GnRH可用于有效治疗山羊卵巢卵泡囊肿，成功率为80%。(C)2004年爱思唯尔公司All rights reserved.
Cystic ovarian disease is an important cause of reproductive failure. The objective of this study was to evaluate transrectal ultrasonography as a diagnostic tool and gonadotropin-releasing hormone (GnRH) as a therapeutic approach for ovarian follicular cysts in goats. Goats were considered to have a follicular cyst(s) if a non-echoic structure >10 mm in diameter was detected in the absence of corpora lutea (CL) in three ultrasonic examinations performed at 5-day intervals. After diagnosis (Day 0), goats with ovarian follicular cysts (n = 5) were treated with a single bolus injection of 10.5 mug synthetic GnRH followed by administration of 125 mug prostaglandin F2alpha (PGF2alpha) 10 days later. Five blood samples were collected at 5-day intervals for determination of progesterone and estradiol-17beta. For detection of LH surge, blood samples were collected every 2 h. Ovulation rate was determined and pregnancy was confirmed by transrectal ultrasonography. The results showed that transrectal ultrasonography is reliable for diagnosis of ovarian follicular cysts and the mean diameter of the follicular cysts was 12.6 +/- 0.4 mm. Plasma concentrations of progesterone and estradiol-17beta at the time of diagnosis of follicular cysts (Day 0) were 0.7 +/- 0.2 ng/ml and 12.7 +/- 0.9 pg/ml, respectively. The concentration of progesterone increased to 4.0 +/- 0.5 ng/ml 10 days after administration of GnRH indicating luteinization of the ovarian follicular cysts concomitant with a decrease in the concentration of estradiol-17beta (3.5 +/- 0.4 pg/ml). Administration of GnRH to cystic goats resulted in a surge of LH within 2 h of treatment. The interval from PGF2alpha injection to the preovulatory LH surge was 62.8 +/- 1.4 h.All goats exhibited estrus 55.2 +/- 2.3 h after PGF2alpha injection and four goats out of the five ovulated. The ovulation rate was 1.5 +/- 0.3. In conclusion, results of this study suggest that transrectal ultrasonography is a reliable tool for diagnosis of ovarian follicular cysts. In addition, GnRH can be used to effectively treat ovarian follicular cysts in goats with 80% success rate. (C) 2004 Elsevier Inc. All rights reserved.