Plant low-molecular-weight phospholipase A2s (PLA2s) are structurally related to the animal secretory PLA2s and are present as a family of isoforms in rice (Oryza sativa)
Plant low-molecular-weight phospholipase A2s (PLA2s) are structurally related to the animal secretory PLA2s and are present as a family of isoforms in rice (Oryza sativa)
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植物低分子量磷脂酶 A2 (PLA2) 在结构上与动物分泌性 PLA2 相关，并且在水稻 (Oryza sativa) 中以异构体家族的形式存在
作者：
U. Stahl;Michael Lee;Staffan Sjödahl;D. Archer;F. Cellini;B. Ek;R. Iannacone;D. Mackenzie;Lucia Semeraro;E. Tramontano;S. Stymme
最近，我们从发育中的榆树种子胚乳中纯化并鉴定了一种低分子量钙依赖性磷脂酶A2 （PLA2）。这是首次从植物组织中纯化和表征PLA2。本文给出了两个不同但同源的水稻（Oryza sativa） cdna的完整序列。这些成熟蛋白编码119个氨基酸（PLA2-I，前面有一个19个氨基酸的信号肽）和128个氨基酸（PLA2-II，前面有一个25个氨基酸的信号肽），来自4个表达序列标签（EST）克隆。这两个蛋白均同源于榆树PLA2的n端氨基酸序列。它们含有12个保守的半胱氨酸残基和序列，可能代表Ca2+结合环和活性位点基序，这是动物分泌PLA2s的特征。从绿色水稻芽中纯化出可溶性PLA2活性14.5万倍。这与榆树和动物分泌PLA2s具有相同的生化特性。纯化后的水稻PLA2由两个分子量为12 440和12 920的蛋白组成，它们具有相同的n端氨基酸序列。该序列与PLA2-I和PLA2-II序列不同，但具有同源性。综上所述，结果表明至少有三种不同的低分子量PLA2s在绿色水稻芽中表达。Southern blot分析表明，这些基因的多个拷贝可能出现在水稻和其他植物基因组中。
Recently, we purified to homogeneity and characterized a low-molecular-weight calcium-dependent phospholipase A2 (PLA2) from developing elm seed endosperm. This represented the first purified and characterized PLA2 from a plant tissue. The full sequences of two distinct but homologous rice (Oryza sativa) cDNAs are given here. These encode mature proteins of 119 amino acids (PLA2-I, preceded by a 19 amino acid signal peptide) and 128 amino acids (PLA2-II, preceded by a 25 amino acid signal peptide), and were derived from four expressed sequence tag (EST) clones. Both proteins were homologous to the N-terminal amino acid sequence of the elm PLA2. They contained twelve conserved cysteine residues and sequences that are likely to represent the Ca2+-binding loop and active-site motif, which are characteristic of animal secretory PLA2s. A soluble PLA2 activity was purified 145 000-fold from green rice shoots. This had the same biochemical characteristics as the elm and animal secretory PLA2s. The purified rice PLA2 consisted of two proteins, with a molecular weight of 12 440 and 12 920, that had identical N-terminal amino acid sequences. This sequence was different from but homologous to the PLA2-I and PLA2-II sequences. Taken together, the results suggest that at least three different low-molecular-weight PLA2s are expressed in green rice shoots. Southern blot analysis suggested that multiple copies of such genes are likely to occur in the rice and in other plant genomes.
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DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chen,J;Engle,SJ;Seilhamer,JJ;Tischfield,JA
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DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Heinrikson,RL
影响因子：
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作者：
Annand, RR;Kontoyianni, M;Gelb, MH
通讯作者：
Gelb, MH
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DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Renetseder,R;Brunie,S;Dijkstra,BW;Drenth,J;Sigler,PB
通讯作者：
Sigler,PB