Cell Culture of Spermatogenic Cells from Amphibians
Cell Culture of Spermatogenic Cells from Amphibians
复制标题
两栖动物生精细胞的细胞培养
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
S. Abe
中科院分区：
文献类型：
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作者：
S. Abe
日本蝾螈（Cynops pyrrhogaster, 1 - 4,10,11）和非洲爪蟾（Xenopus laevis, 2,3,5,17,18）已经建立了从初级精母细胞阶段到早期、中期精母细胞阶段的生精细胞培养。许多研究结果表明，每个物种的精子在体外的成熟过程与在体内是沿着相同的途径和相同的时间控制进行的。由于这两个物种的成熟精子的形状和大小非常不同，比较研究将使我们能够阐明精子形态决定的机制。从体外精子发生研究中获得的结果表明，独爪蟾和非洲爪蟾具有共同的和物种特异性的特征。
Cell culture of spermatogenic cells from the primary spermatocyte stage to the early, midspermatid stage has been established for the Japanese newt, Cynops pyrrhogaster (1–4, 10, 11) and for Xenopus laevis (2, 3, 5, 17, 18). The results of many investigations suggest that sperm maturation in vitro for each species proceeds along the same pathway and under the same temporal control as in vivo. Since the shape and size of mature sperm of these two species are very different, comparative studies would enable us to elucidate the mechanisms by which sperm morphology is determined. The results obtained from in vitro studies of spermatogenesis in Cynops and Xenopus demonstrate that there are common and species‐specific features.
在无血清培养基中培养的非洲爪蟾睾丸外植体中精子发生的体外维持。
影响因子：
3.6
作者：
Risley,MS;Miller,A;Bumcrot,DA
通讯作者：
Bumcrot,DA