In Vitro Survival of Human Mesenchymal Stem Cells is Enhanced in Artificial Endolymph with Moderately High Concentrations of Potassium
In Vitro Survival of Human Mesenchymal Stem Cells is Enhanced in Artificial Endolymph with Moderately High Concentrations of Potassium
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DOI：
10.1089/scd.2018.0016
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发表时间：
2018
影响因子：
4
通讯作者：
Robert Chunhua Zhao 赵春华
中科院分区：
文献类型：
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作者：
Juanjuan Gao 高娟娟;Shihua Wang 王世华;Qi Tang 唐琦;Xiaoxia Li;Yongli Zhang 张永丽;Wenbin Liu 刘文斌;Zhiqiang Gao 高志强;Hua Yang 杨华;Robert Chunhua Zhao 赵春华
While mesenchymal stem cells (MSCs) are promising candidates for inner ear hair cell regeneration, to date, there have been no convincing reports indicating whether MSCs can survive in the cochlea for more than a few weeks, as the high levels of potassium (K+) in the endolymph (EL) are thought to be toxic to transplanted stem cells. For conditioning the EL for MSC transplantation, we conducted this in vitro study to examine the effects of artificial EL with altered K+ concentration levels, in the range of 5–153.8 mM, on proliferation, apoptosis, and morphological changes in MSCs derived from various human tissues. Our findings demonstrate that altering the K+ concentration in artificial EL could significantly influence the survival of MSCs in vitro. We discovered.that K+ concentrations of 55–130mM in artificial EL could enhance the survival of MSCs in vitro. However, MSCs exhibited reduced proliferation regardless of K+ concentration.
