Scanning Force Microscopic Studies of Escherichia coli Ribosomes on Solid Substrate Surface
Scanning Force Microscopic Studies of Escherichia coli Ribosomes on Solid Substrate Surface
复制标题
固体基质表面大肠杆菌核糖体的扫描力显微镜研究
DOI：
10.1143/jjap.43.4599
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发表时间：
2004
影响因子：
1.5
通讯作者：
T. Kawai
中科院分区：
文献类型：
--
作者：
T. Matsuura;K. Kobayashi;Hiroyuki Tanaka;T. Matsumoto;T. Kawai
使用扫描力显微镜 (SFM) 对从大肠杆菌中分离出的核糖体和核糖体亚基进行成像。直接获得云母表面吸附的核糖体颗粒的形貌图像。在 80 nM 时，核糖体聚集。在 ≤8 nM 时，实现了稀疏覆盖，单个核糖体彼此分离。尽管获得的高度明显低于X射线衍射测量的直径，但高度直方图显示观察到的核糖体颗粒属于三种高度分布：大约4、9和11 nm。这三个分布分别对应于 70S 核糖体、50S 和 30S 核糖体亚基。这项工作表明，SFM 可用于区分固体基质表面上的极少量核糖体颗粒。
Scanning force microscopy (SFM) was used to image ribosomes and ribosomal subunits isolated from Escherichia coli. Topographic images of ribosomal particles adsorbed on a mica surface were obtained directly. At 80 nM, the ribosomes aggregated. At ≤8 nM, a sparser coverage was achieved, with single ribosomes isolated from one another. Although the obtained height was significantly lower than the diameter measured by X-ray diffraction, the height histogram revealed that the observed ribosomal particles belong to three height distributions: approximately 4, 9 and 11 nm. These three distributions correspond to the 70S ribosome, and the 50S and 30S ribosomal subunits, respectively. This work demonstrates that SFM is useful in the discrimination of very small amounts of ribosomal particles on a solid substrate surface.