HUMAN-ENDOTHELIAL CELLS - USE OF HEPARIN IN CLONING AND LONG-TERM SERIAL CULTIVATION
HUMAN-ENDOTHELIAL CELLS - USE OF HEPARIN IN CLONING AND LONG-TERM SERIAL CULTIVATION
复制标题
DOI：
10.1126/science.6635659
复制
发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
LEVINE, EM
中科院分区：
文献类型：
--
作者：
THORNTON, SC;MUELLER, SN;LEVINE, EM
体外培养人血管内皮细胞，倍增时间为17 ~ 21 h，倍增时间为42 ~ 79倍。首次建立了人内皮细胞克隆株，并具有相似的增殖能力。通过向含有降低浓度的内皮细胞生长因子的培养基中加入肝素来实现这种旺盛的细胞生长。人内皮细胞的常规克隆和长期培养将为人内皮细胞的体外研究奠定基础。
Endothelial cells from human blood vessels were cultured in vitro, with doubling times of 17 to 21 h for 42 to 79 population doublings. Cloned human endothelial cell strains were established for the 1st time and had similar proliferative capacities. This vigorous cell growth was achieved by addition of heparin to culture medium containing reduced concentrations of endothelial cell growth factor. The routine cloning and long-term culture of human endothelial cells will facilitate studying the human endothelium in vitro.