Endothelium-dependent vasorelaxation effect of rutin-free tartary buckwheat extract in isolated rat thoracic aorta.
Endothelium-dependent vasorelaxation effect of rutin-free tartary buckwheat extract in isolated rat thoracic aorta.
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DOI：
10.1016/j.jnutbio.2007.09.005
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发表时间：
2008-10
期刊：
影响因子：
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通讯作者：
Yusuke Ushida;T. Matsui;Mitsuru Tanaka;Kiyoshi Matsumoto;H. Hosoyama;A. Mitomi;Y. Sagesaka;T. Kakuda
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文献类型：
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作者：
Yusuke Ushida;T. Matsui;Mitsuru Tanaka;Kiyoshi Matsumoto;H. Hosoyama;A. Mitomi;Y. Sagesaka;T. Kakuda
本研究的目的是指出苦荞对血管功能的潜力。采用SP70色谱柱（TBSP-T）对苦荞热水浸提液中不含芦丁的非吸收部分进行研究。在大鼠胸主动脉环经1.0 μM苯肾上腺素（PE）或50 mM KCl收缩的收缩实验中，TBSP-T引起血管松弛[EC50(mg/ml): PE；2.2;氯化钾,1.9]。进一步将TBSP-T液液分离为碱性、中性和酸性组分，发现只有酸性组分（EC50, 0.25 mg/ml）有明显的血管舒张作用。在内皮剥脱的主动脉环和一氧化氮合酶抑制剂ng - monom甲基-l-精氨酸（100 μM）存在下，酸性组分的作用明显减弱。cGMP （pmol/mg蛋白）：PE, 7.2±2.3；PE+酸性组分，35±8]。这些结果表明，酸性部分可以介导NO/cGMP通路，从而发挥内皮依赖性血管舒张作用。综上所述，苦荞具有调节血管张力的作用，除芦丁外，还具有潜在的酸性候选物质。
The aim of this study was to point out the potential of tartary buckwheat on vascular functions. A nonabsorbed fraction of hot-water extract of tartary buckwheat on a SP70 column (TBSP-T), which was free from rutin, was used for this aim. In a contractile experiment using Sprague–Dawley rat thoracic aorta rings contracted by 1.0 μM phenylephrine (PE) or 50 mM KCl, TBSP-T evoked a significant vasorelaxation [EC50(mg/ml): PE; 2.2; KCl, 1.9]. By a further fractionation of TBSP-T by liquid–liquid partitioning into basic, neutral and acidic fractions, a marked enhancement of vasorelaxation effect was observed only for acidic fraction (EC50, 0.25 mg/ml). The action of acidic fraction was significantly attenuated in endothelium-denuded aortic rings and in the presence of nitric oxide synthase inhibitor, NG-monomethyl-l-arginine (100 μM). The fraction also enhanced the cyclic guanosine monophosphate (cGMP) production in aortic rings contracted with PE [cGMP (pmol/mg protein): PE, 7.2±2.3; PE+Acidic fraction, 35±8]. These results indicate that acidic fraction could mediate NO/cGMP pathways, thereby exerting endothelium-dependent vasorelaxation action. In conclusion, tartary buckwheat was proven to regulate vascular tones and have latent acidic candidates except for rutin.