STRUCTURE AND EXPRESSION OF THE MOUSE MYELIN PROTEOLIPID PROTEIN GENE
STRUCTURE AND EXPRESSION OF THE MOUSE MYELIN PROTEOLIPID PROTEIN GENE
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DOI：
10.1002/jnr.490180302
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发表时间：
1987-01-01
影响因子：
4.2
通讯作者：
GARDINIER, MV
中科院分区：
文献类型：
--
作者：
MACKLIN, WB;CAMPAGNONI, CW;GARDINIER, MV
小鼠髓鞘蛋白脂蛋白的基因已经被分离出来，并对其七个外显子进行了测序。由于大鼠蛋白脂蛋白基因的cDNA序列和人类蛋白脂蛋白基因的部分序列已经确定，因此蛋白脂蛋白基因外显子在物种之间可能具有很高的保守性。虽然有一些核苷酸变化，但小鼠基因的蛋白质编码区编码的蛋白质相对于大鼠和人类蛋白脂蛋白是完全保守的。蛋白质脂蛋白基因的调节区和非编码区也是高度保守的。该基因的上游调控区和5‘非编码区与人蛋白脂蛋白基因的可比区同源性为92%，而小鼠基因的3’非编码区与大鼠蛋白脂蛋白基因的2,200个核苷酸的3‘非编码区的同源性约为90%。S1核酸酶保护实验表明，小鼠、大鼠、人和狒狒的蛋白质脂蛋白mRNAs的主要5‘端位于蛋白质编码区起始蛋氨酸密码子上游约147-160个核苷酸。其他的S1核酸酶保护实验表明，在外显子3中可能存在一个选择性剪接位点，它可能产生DM20的mRNA。该基因比蛋白脂蛋白短约100个核苷酸，缺失外显子3的后半部分，即蛋白脂蛋白序列的116-150个氨基酸。
The gene for the mouse myelin proteolipid protein has been isolated and the seven exons have been sequenced. Since the sequence of a rat proteolipid protein cDNA and partial sequence of the human proteolipid protein gene have been determined, it was possible to demonstrate a very high degree of conservation for the proteolipid protein gene exons among species. While there are some nucleotide changes, the protein coding region of the mouse gene encodes protein that is totally conserved relative to both rat and human proteolipid proteins. The regulatory and non-coding regions of the proteolipid protien gene are also highly conserved. The upstream regulatory and 5''-noncoding region of the gene is 92% homologous to the comparable region of the human proteolipid protein gene, and the 3''-noncoding region of the mouse gene is approximately 90% homologous to a rat proteolipid protein cDNA through 2,200 nucleotides of 3''-noncoding DNA. S1 nuclease protection experiments indicated that the major 5''-end for proteolipid protein mRNAs from mouse, rat, human, or baboon is approximately 147-160 nucleotides upstream from the initial methionine codon of the protein coding region. Other S1 nuclease protection experiments indicated the possible existence of an alternative splice site within exon 3, which may produce mRNA for DM20. This mRNA is approximately 100 nucleotides shorter than that for the proteolipid protein, and it is missing the latter half of exon 3, that is, amino acids 116-150 of the proteolipid protein sequence.